CS0170

Thioredoxin Reductase Assay Kit

1 kit sufficient for 100 assays (1 mL)

Manufacturer: Sigma Aldrich

Synonym(S): Thioredoxin Reductase Activity Kit

Select a Size

Pack Size SKU Availability Price
1 KIT CS0170-1-KIT In Stock ₹ 63,258.90

CS0170 - 1 KIT

₹ 63,258.90

In Stock

Quantity

1

Base Price: ₹ 63,258.90

GST (18%): ₹ 11,386.602

Total Price: ₹ 74,645.502

Quality Level

200

usage

kit sufficient for 100 assays (1 mL)

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... TXNRD1(7296) , TXNRD2(10587) , TXNRD3(114112)

Description

  • General description: The Thioredoxin Reductase Assay Kit uses a colorimetric assay for the determination of thioredoxin reductase activity in mammalian cells and tissues. It is based on the reduction of 5,5′-dithiobis(2-nitrobenzoic) acid (DTNB) with NADPH to 5-thio-2-nitrobenzoic acid (TNB) that produces a strong yellow color that is measured at 412 nm. DTNB + NADPH + H+ ↔ 2 TNB + NADP+
  • Application: Using post nuclear extracts of rat liver, the kit shows linearity up to 0.25 units per mL. In this preparation, ~20% of the activity is due to non-specific reductase activity (refractory to the TrxR inhibitor).
  • Features and Benefits: An easy and simple methodThe kit contains all the reagents required for a colorimetric assay of mammalian thioredoxin reductase The kit includes an inhibitor solution for specific inhibition of mammalian thioredoxin reductaseThe kit has been tested with heart muscle, as well as lysates from cell lines such as HeLa, A549, Jurkat, U937, A431, COS, CHO, and NIH 3T3 cells

SAFETY INFORMATION

Pictograms

GHS08

Signal Word

Warning

Hazard Statements

H361

Precautionary Statements

P201 - P202 - P280 - P308 + P313 - P405 - P501

Hazard Classifications

Repr. 2

WGK

WGK 3

Flash Point(F)

188.6 °F

Flash Point(C)

87 °C

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