Description

• General description: Guanidine hydrochloride, also known as aminoformamidine hydrochloride, is a versatile compound widely used in molecular biology and biochemical research. Functioning as a potent chaotropic agent, it disrupts protein and nucleic acid structures, making it invaluable for purifying proteins, particularly mRNA. Its denaturing capabilities extend to unfolding proteins, aiding their purification and subsequent refolding, and it can even solubilize denatured insoluble proteins at higher concentrations.At lower concentrations, guanidine hydrochloride exhibits a unique effect, promoting the refolding of denatured proteins and restoring enzymatic activity. This property is advantageous for protein renaturation studies. In RNA extraction, guanidine hydrochloride acts as a robust denaturant, disrupting cell structures and inactivating RNA enzymes, ensuring the integrity of extracted RNA. In summary, guanidine hydrochloride is a versatile compound with applications in protein purification, nucleic acid isolation, and protein refolding studies, showcasing its multifaceted utility in molecular biology and biochemical research.
• Application: Guanidine hydrochloride has been used: as a component of the extraction buffer for the extraction of proteoglycans[1]in extraction during protein fractionation of ATDC5 cell lines[2]as a chemical additive to study its effective absorbance spectra in structural analysis[3] to incubate the device chip to release prostate-specific antigen and for regeneration of aptamer[4]as a component of the 2,4-dinitrophenylhydrazine (DNPH) solution for detecting protein carbonyls by protein carbonylation study[5]in RNA isolation to dissociate nucleoproteins and inhibit RNase
• Biochem/physiol Actions: Guanidine hydrochloride (GuHCl) is a small hydroscopic molecule.[6][7] It plays a role in inhibiting heat shock protein 104 (Hsp104) adenosine triphosphatase (ATPase) activity in vivo.[6] It is a potent denaturant and inactivator of several enzymes and proteins.[7][8] GuHCl can inactivate aminoacylase and papain.[8] It is involved in unfolding and inactivation of alkaline phosphatase in Haliotis diversicolor.[8] At higher concentrations, GuHCl is involved in virus inactivation such as Herpes simplex virus 1 (HSV-1).[9]
• Features and Benefits: Highly versatile surfactant for your cell biology and biochemical researchSuitable for sensitive molecular biology applicationsFree from Exonuclease, Dnase and RnaseTested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
• Caution: May agglomerate upon storage. The quality of the product does not appear to be affected and solutions prepared from the free-flowing and lumpy guanidine hydrochloride appear identical.
• Preparation Note: In order to make an 8M solution in water, one must heat the solution to 35 °C for approximately 30 minutes. The maximum solubility of guanidine hydrochloride in water at room temperature is approximately 6M.
• Other Notes: For additional information on our range of Biochemicals, please complete this form.