MAK337
Aconitase Assay Kit
sufficient for 100 colorimetric tests
Manufacturer: Sigma Aldrich
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 KIT | MAK337-1-KIT | In Stock | ₹ 40,914.60 |
MAK337 - 1 KIT
In Stock
Quantity
1
Base Price: ₹ 40,914.60
GST (18%): ₹ 7,364.628
Total Price: ₹ 48,279.228
usage
sufficient for 100 colorimetric tests
input
tissue homogenate(s) cell lysate serum
detection method
colorimetric
relevant disease(s)
endocrinological disorders, diabetes; neurological disorders; rheumatological diseases
storage temp.
−20°C
Related Products
Description
- General description: Aconitase (Aconitate Hydratase) is an enzyme in the citric acid (TCA) and glyoxylate cycles that catalyzes the conversion of citrate to isocitrate. The activity of aconitase depends largely upon the iron-sulfur [Fe⁴S⁴] 2+ cluster. Related diseases include aconitase deficiency (such as myopathy and exercise intolerance. Friedreich′s ataxia and diabetes.) Mitochondrial aconitase also plays a role in stabilizing mtDNA, thereby influencing mitochondrial gene expression. Aconitase serves as a reliable marker of mitochondrial and cellular oxidative stress. The alteration of mitochondrial aconitase can lead to reduced energy production. The cytosolic form can act as an aconitase and also as an iron regulatory protein. The enzyme′s activity is inhibited by citrate analogs and fluoracetate. Oxidative stress agents like peroxynitrite, hydrogen peroxide, and superoxide can deactivate the enzyme by converting the [Fe⁴S⁴] cluster to a [Fe3S⁴] cluster.
- Application: The Aconitase Assay Kit may be used for:Cellular and Mitchondrial Metabolism ResearchOxidative Stress ResearchNeurological Research
- Features and Benefits: Fast and Sensitive Measurement: Achieve quantitative measurement of aconitase activity using just 20 µL of sample, with a detection range spanning from 0.5 to 100 U/L.High-Throughput Compatibility: Seamlessly integrate the kit into high-throughput handling systems, ensuring efficiency and accuracy in assays.
- Suitability: Suitable for aconitase activity determination in biological samples (e.g. cell lysate, tissue homogenate, serum, etc.)
- Principle: The Aconitase Assay Kit measures the isocitrate generated as a product of the aconitase reaction. The isocitrate is then oxidized producing NADPH and the oxidation product. The NADPH converts the dye to an intense violet color with an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to aconitase activity.
- Other Notes: For additional information on our range of Biochemicals, please complete this form.
SAFETY INFORMATION
Pictograms
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
WGK
WGK 3
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usage: sufficient for 100 colorimetric tests
input: tissue homogenate(s) cell lysate serum
detection method: colorimetric
relevant disease(s): endocrinological disorders, diabetes; neurological disorders; rheumatological diseases
storage temp.: −20°C
usage:
sufficient for 100 colorimetric tests
input:
tissue homogenate(s) cell lysate serum
detection method:
colorimetric
relevant disease(s):
endocrinological disorders, diabetes; neurological disorders; rheumatological diseases
storage temp.:
−20°C
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plasma food(s) (oil) blood tissue extract(s) food(s) serum
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relevant disease(s):
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detection method: __
relevant disease(s): __
storage temp.: −20°C
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detection method: fluorometric
relevant disease(s): cardiovascular diseases
storage temp.: __
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sufficient for 100 fluorometric tests
input:
__
detection method:
fluorometric
relevant disease(s):
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