PPB002

Tris Buffered Saline

powder, pH 8.0, pHast Pack™, with 0.05% Tween® 20

Manufacturer: Sigma Aldrich

Synonym(S): Tris Buffered Saline, with Tween™ 20, pH 8.0, TBS with 0.05% Tween-20, TBS-T, TBST

Select a Size

Pack Size SKU Availability Price
20 PKG PPB002-20-PKG In Stock ₹ 9,212.08

PPB002 - 20 PKG

₹ 9,212.08

In Stock

Quantity

1

Base Price: ₹ 9,212.08

GST (18%): ₹ 1,658.174

Total Price: ₹ 10,870.254

product name

Tris Buffered Saline, with 0.05% Tween® 20, pH 8.0, powder, pHast Pack™

product line

pHast Pack™

form

powder

storage condition

(Tightly closed. Dry.)

impurities

≤5 ppm Heavy Metals (lead)

color

white

pH

7.8—8.2

solubility

soluble, clear to hazy, colorless

cation traces

Fe: ≤10 ppm

foreign activity

DNase, none detectedEndonuclease Exonuclease, none detectedNICKase, none detectedProtease, none detectedRNase, none detected

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Description

  • General description: Tris Buffered Saline with 0.05% Tween® 20 or TBST has a low concentration of detergent with a slightly basic pH of 8.0. It is a commonly used washing buffer alternative to PBS-T in many immunodetection techniques. It decreases non-specific bindings from the western blot membrane and microtiter plate walls.
  • Application: Suitable for use as a wash buffer in:Western blotELISAImmunohistochemistry
  • Features and Benefits: Ready-to-use buffer requires no measurments or pH adjustmentsSaves time, money and effort to get your assays started fastPhysical, chemical, and biological tests for quality: free of DNase, RNase, Protease, and NickaseQualified for use in gel electrophoreis and other molecular biology assays
  • Packaging: Foil pouches
  • Reconstitution: Contents of one pouch, when dissolved in 500 mL of distilled or deionized water, will yield a 1× solution containing 50 mM Tris, 138 mM NaCl, 2.7 mM KCl; 0.05%Tween 20 (w/v), pH 8.0 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free.
  • Storage and Stability: Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.
  • Other Notes: For additional information on our range of Biochemicals, please complete this form.
  • Legal Information: TWEEN is a registered trademark of Croda International PLC

SAFETY INFORMATION

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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product name:
Tris Buffered Saline, with 0.05% Tween® 20, pH 8.0, powder, pHast Pack™

product line:
pHast Pack™

form:
powder

storage condition:
(Tightly closed. Dry.)

impurities:
≤5 ppm Heavy Metals (lead)

color:
white

pH:
7.8—8.2

solubility:
soluble, clear to hazy, colorless

cation traces:
Fe: ≤10 ppm

foreign activity:
DNase, none detectedEndonuclease Exonuclease, none detectedNICKase, none detectedProtease, none detectedRNase, none detected

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form:
powder

storage condition:
(Tightly closed. Dry)

impurities:
≤5 ppm Heavy Metals (lead)

color:
white

pH:
7.2—7.6 (25 °C)

solubility:
soluble, clear to hazy, colorless

cation traces:
Fe: <10 ppm

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