Phosphate buffered saline

Description

• General description: Phosphate buffered saline or PBS, pH 7.4 is a commonly used biological buffer in molecular biology. A 1X working solution is typically used in cell culture and electrophoresis assays to wash away non-adherent cells or any unbound biomolecules. PBS is hypo-osmotic and can be harmful to cells, causing swelling and resulting in cell lysis, if left for extended periods.
• Application: Phosphate buffered saline is commonly used in washing solutions for: ELISA Western Blotting and other protein applications Cell culture (sterile filtration recommended) Resuspension and sonication of cells Immunohistochemistry
• Features and Benefits: Save time with our ready-to-use formulation No calculations, measurements, and adjusting pH Reliable quality for reproducibility Suitable for use in cell culture and electrophoresis Biological tests: free of DNase, RNase, Protease, and Nickase Chemical tests: Iron ≤10 ppm, Lead ≤5 ppm
• Packaging: Foil pouches
• Reconstitution: Contents of one pouch, when dissolved in 500mL of distilled or deionized water, will yield a 1X solution containing 10mM Na2HPO4, 1.8mM KH2PO4, 138mM NaCl, 2.7mM KCl, pH 7.4 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free. Certified for use in cell culture.
• Storage and Stability: Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.
• Other Notes: For additional information on our range of Biochemicals, please complete this form.
• Legal Information: pHast Pack is a trademark of Sigma-Aldrich Co. LLC