Tris buffer

Description

• General description: Tris buffer, pH 8.1 is a general biological buffer commonly used in biochemistry and molecular biology. It is isotonic and non-toxic in nature due to the presence of tris base. Tris has a buffering capacity range of pH 7.0 - 9.0, with a pKa of 8.1 at 25°C.
• Application: Intended for use in: Cell culture (sterile filter recommended) Western blot Protein electrophoresis DNA agarose electrophoresis as a component to make Tris acetate-EDTA (TAE) and Tris borate-EDTA (TBE) buffers
• Features and Benefits: Ready-to-use for quick buffer prep No measuring and adjusting pH Saves time and minimizes effort in the lab Biological tests: free of DNase, RNase, protease, and nickase Chemical tests: Iron ≤10 ppm, lead ≤5 ppm
• Packaging: Foil pouches
• Reconstitution: Contents of one pouch, when dissolved in 500 mL of ultrapure water, will yield a 0.1M Tris solution, pH 8.1 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free. This product is suitable for use in cell culture and electrophoresis applications.
• Storage and Stability: Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.
• Other Notes: For additional information on our range of Biochemicals, please complete this form.
• Legal Information: pHast Pack is a trademark of Sigma-Aldrich Co. LLC