Tris-Glycine Buffer

Description

• General description: Tris-Glycine buffer is primarily used as a running buffer and to prepare transfer buffer in western blot analysis with the addition of methanol. This buffer can separate proteins by their size. It has a pH of 8.3.
• Application: Suitable for use as: Transfer buffer component in western blot Running buffer in native gel electrophoresis for protein separation Running buffer in gradient polyacrylamide gel electrophoresis (PAGE)
• Features and Benefits: Ready-to-use buffer No measuring and pH adjusting needed Saves time and minimizes the effort Biological tests: free of DNase, RNase, protease, and nickase Chemical tests: Iron ≤10 ppm, lead ≤5 ppm
• Packaging: Foil pouches
• Reconstitution: Contents of one pouch, when dissolved in 500 mL of ultrapure water, will yield a 1x solution containing 25 mM Tris, 192 mM Glycine, pH 8.3 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free.
• Storage and Stability: Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.
• Other Notes: For additional information on our range of Biochemicals, please complete this form.
• Legal Information: pHast Pack is a trademark of Sigma-Aldrich Co. LLC