PPB008
Tris Acetate-EDTA buffer
pH 8.3, pHast Pack™, powder
Manufacturer: Sigma Aldrich
Synonym(S): 1X TAE buffer, TAE, Tris Acetate EDTA, TAE buffer
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 20 PKG | PPB008-20-PKG | In Stock | ₹ 9,956.70 |
PPB008 - 20 PKG
In Stock
Quantity
1
Base Price: ₹ 9,956.70
GST (18%): ₹ 1,792.206
Total Price: ₹ 11,748.906
product line
pHast Pack™
form
powder
pH
8.3
suitability
suitable for gel electrophoresis (after dilution to working concentration)suitable for gel electrophoresis
foreign activity
DNAse, none detectedNickase, none detectedProtease, none detectedRNAse, none detected
SMILES string
CC(O)=O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
InChI
1S/C10H16N2O8.C4H11NO3.C2H4O2/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;1-2(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;1H3,(H,3,4)
InChI key
HGEVZDLYZYVYHD-UHFFFAOYSA-N
Related Products
Description
- General description: Tris Acetate-EDTA (TAE) buffer, with a pH range of 8.1 – 8.4, is typically used for agarose gel electrophoresis, especially for the recovery of DNA. For larger double-stranded DNA fragments (>12kB) TAE buffer is preferred. However, the buffering capacity can become exhausted if electrophoresis time is extended. Buffer circulation or replacement during electrophoresis can remedy the lower buffering capacity.
- Application: Suitable as a running and gel preparation buffer in: DNA agarose gel electrophoresis Non-denaturing RNA agarose gel electrophoresis for RNA >1500 bases Denaturing gradient gel electrophoresis Northern and Southern blotting
- Features and Benefits: Ready for use – saves time and effort No measuring and pH adjusting needed Eliminate exposure to toxic chemicals to prepare buffersBiological tests: free of DNase, RNase, Protease, and Nickase Chemical tests: Iron ≤10 ppm, lead ≤5 ppm
- Packaging: Foil pouches
- Preparation Note: Prepared with Biotechnology Performance Certified Trizma base (T6066) and Molecular Biology Reagent EDTA disodium salt (E5134). Solutions also contain acetic acid (A6283); powdered blend contains Trizma acetate (T1258).
- Reconstitution: Contents of one pouch, when dissolved in 500mL of distilled or deionized water, will yield a 1X solution containing 40mM Tris-Acetate, 1mM EDTA, pH 8.3 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free. Certified for use in electrophoresis.
- Storage and Stability: Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.
- Other Notes: For additional information on our range of Biochemicals, please complete this form.
- Legal Information: pHast Pack is a trademark of Sigma-Aldrich Co. LLC
SAFETY INFORMATION
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Compare Similar Items
Show Difference
product line: pHast Pack™
form: powder
pH: 8.3
suitability: suitable for gel electrophoresis (after dilution to working concentration)suitable for gel electrophoresis
foreign activity: DNAse, none detectedNickase, none detectedProtease, none detectedRNAse, none detected
SMILES string: CC(O)=O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
InChI: 1S/C10H16N2O8.C4H11NO3.C2H4O2/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;1-2(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;1H3,(H,3,4)
InChI key: HGEVZDLYZYVYHD-UHFFFAOYSA-N
product line:
pHast Pack™
form:
powder
pH:
8.3
suitability:
suitable for gel electrophoresis (after dilution to working concentration)suitable for gel electrophoresis
foreign activity:
DNAse, none detectedNickase, none detectedProtease, none detectedRNAse, none detected
SMILES string:
CC(O)=O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
InChI:
1S/C10H16N2O8.C4H11NO3.C2H4O2/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;1-2(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;1H3,(H,3,4)
InChI key:
HGEVZDLYZYVYHD-UHFFFAOYSA-N
product line: pHast Pack™
form: powder
pH: 8.3
suitability: suitable for gel electrophoresis
foreign activity: DNAse, none detectedNickase, none detectedProtease, none detectedRNAse, none detected
SMILES string: OB(O)O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
InChI: 1S/C10H16N2O8.C4H11NO3.BH3O3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;2-1(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;2-4H
InChI key: OSBLTNPMIGYQGY-UHFFFAOYSA-N
product line:
pHast Pack™
form:
powder
pH:
8.3
suitability:
suitable for gel electrophoresis
foreign activity:
DNAse, none detectedNickase, none detectedProtease, none detectedRNAse, none detected
SMILES string:
OB(O)O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
InChI:
1S/C10H16N2O8.C4H11NO3.BH3O3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;2-1(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;2-4H
InChI key:
OSBLTNPMIGYQGY-UHFFFAOYSA-N
product line: pHast Pack™
form: powder
pH: 7.9—8.1
suitability: __
foreign activity: DNAse, none detectedNickase, none detectedProtease, none detectedRNAse, none detected
SMILES string: __
InChI: __
InChI key: __
product line:
pHast Pack™
form:
powder
pH:
7.9—8.1
suitability:
__
foreign activity:
DNAse, none detectedNickase, none detectedProtease, none detectedRNAse, none detected
SMILES string:
__
InChI:
__
InChI key:
__
product line: pHast Pack™
form: powder
pH: 7.6
suitability: __
foreign activity: __
SMILES string: __
InChI: __
InChI key: __
product line:
pHast Pack™
form:
powder
pH:
7.6
suitability:
__
foreign activity:
__
SMILES string:
__
InChI:
__
InChI key:
__