PPB009

Tris-Borate-EDTA buffer

pH 8.3, pHast Pack™, powder

Manufacturer: Sigma Aldrich

Synonym(S): 1X TBE buffer, TBE buffer, Tris Borate EDTA

Select a Size

Pack Size SKU Availability Price
20 PKG PPB009-20-PKG In Stock ₹ 9,956.70

PPB009 - 20 PKG

₹ 9,956.70

In Stock

Quantity

1

Base Price: ₹ 9,956.70

GST (18%): ₹ 1,792.206

Total Price: ₹ 11,748.906

product line

pHast Pack™

form

powder

pH

8.3

suitability

suitable for gel electrophoresis

foreign activity

DNAse, none detectedNickase, none detectedProtease, none detectedRNAse, none detected

SMILES string

OB(O)O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8.C4H11NO3.BH3O3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;2-1(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;2-4H

InChI key

OSBLTNPMIGYQGY-UHFFFAOYSA-N

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Description

  • General description: Tris-Borate-EDTA (TBE) buffer is a commonly used running buffer for nucleic gel electrophoresis with a pH range from 8.1-8.5 (25 °C). TBE buffer has a higher buffer capacity than TAE buffer and has a higher resolution for DNA fragments <1500 bp. Borate in TBE buffer acts as an enzyme inhibitor.
  • Application: Suitable as a running and gel preparation buffer in: DNA agarose gel electrophoresis (fragment <1500 bp) Native and non-denaturing RNA agarose gel electrophoresis (fragment <1500 bp) Denaturing gradient gel electrophoresis Northern and Southern blotting DNA automated sequencing gel Pulsed-field gel electrophoresis (PFGE) (applied voltages of less than 5 V/cm are recommended for maximum resolution)
  • Features and Benefits: Ready-to-use buffer No measuring and pH adjusting needed Save time and minimize effort Minimize chemical exposure to toxic chemicals Biological tests: free of DNase, RNase, Protease, and Nickase Chemical tests: Iron ≤10 ppm, lead ≤5 ppm
  • Packaging: Foil pouches
  • Preparation Note: Prepared with Biotechnology Performance Certified Trizma base (Product Code T6066) and Molecular Biology Reagents boric acid (Product Code B6768) and EDTA disodium salt (Product Code E5134).
  • Reconstitution: Contents of one pouch, when dissolved in 500mL of distilled or deionized water, will yield a 1X solution containing 89mM Tris-Borate, 2mM EDTA, pH 8.3 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free. Certified for use in electrophoresis. Recommended temperature for dissolution 5 °C to 40 °C.
  • Storage and Stability: Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.
  • Other Notes: For additional information on our range of Biochemicals, please complete this form.
  • Legal Information: pHast Pack is a trademark of Sigma-Aldrich Co. LLC

SAFETY INFORMATION

Pictograms

GHS08

Signal Word

Danger

Hazard Statements

H360FD

Precautionary Statements

P201 - P202 - P280 - P308 + P313 - P405 - P501

Hazard Classifications

Repr. 1B

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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SMILES string:
OB(O)O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI:
1S/C10H16N2O8.C4H11NO3.BH3O3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;2-1(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;2-4H

InChI key:
OSBLTNPMIGYQGY-UHFFFAOYSA-N

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