Tris-Borate-EDTA buffer

Description

• General description: Tris-Borate-EDTA (TBE) buffer is a commonly used running buffer for nucleic gel electrophoresis with a pH range from 8.1-8.5 (25 °C). TBE buffer has a higher buffer capacity than TAE buffer and has a higher resolution for DNA fragments <1500 bp. Borate in TBE buffer acts as an enzyme inhibitor.
• Application: Suitable as a running and gel preparation buffer in: DNA agarose gel electrophoresis (fragment <1500 bp) Native and non-denaturing RNA agarose gel electrophoresis (fragment <1500 bp) Denaturing gradient gel electrophoresis Northern and Southern blotting DNA automated sequencing gel Pulsed-field gel electrophoresis (PFGE) (applied voltages of less than 5 V/cm are recommended for maximum resolution)
• Features and Benefits: Ready-to-use buffer No measuring and pH adjusting needed Save time and minimize effort Minimize chemical exposure to toxic chemicals Biological tests: free of DNase, RNase, Protease, and Nickase Chemical tests: Iron ≤10 ppm, lead ≤5 ppm
• Packaging: Foil pouches
• Preparation Note: Prepared with Biotechnology Performance Certified Trizma base (Product Code T6066) and Molecular Biology Reagents boric acid (Product Code B6768) and EDTA disodium salt (Product Code E5134).
• Reconstitution: Contents of one pouch, when dissolved in 500mL of distilled or deionized water, will yield a 1X solution containing 89mM Tris-Borate, 2mM EDTA, pH 8.3 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free. Certified for use in electrophoresis. Recommended temperature for dissolution 5 °C to 40 °C.
• Storage and Stability: Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.
• Other Notes: For additional information on our range of Biochemicals, please complete this form.
• Legal Information: pHast Pack is a trademark of Sigma-Aldrich Co. LLC