SBR00022
Blasticidin S Ready Made Solution
10 mg/mL (20 mM HEPES), 0.22 μm filtered
Manufacturer: Sigma Aldrich
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 ML | SBR00022-1-ML | In Stock | ₹ 9,298.68 |
| 10 ML | SBR00022-10-ML | In Stock | ₹ 80,516.35 |
SBR00022 - 1 ML
In Stock
Quantity
1
Base Price: ₹ 9,298.68
GST (18%): ₹ 1,673.762
Total Price: ₹ 10,972.442
Quality Level
200
sterility
0.22 μm filtered
Assay
≥98% (HPLC)
form
liquid
concentration
10 mg/mL (20 mM HEPES)
color
colorless to faint yellow
antibiotic activity spectrum
fungi
Mode of action
protein synthesis | interferes
storage temp.
−20°C
SMILES string
CN(CCC(CC(=O)NC1C=CC(OC1C(=O)O)N2C=CC(=NC2=O)N)N)C(=N)N.Cl
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Description
- General description: Blasticidin S, originally isolated from S. griseochromogenes, is a bacterial metabolite renowned for its antibacterial and fungicidal activities. This compound serves as a potent inhibitor of protein synthesis, exhibiting activity against various microorganisms, including bacteria (B. subtilis, S. lutea, E. coli, P. fluorescens, and M. tuberculosis), tumor cell lines, and nematodes. In research, Blasticidin S has become a valuable tool, notably as a marker for strain manipulations. Recent applications involve the use of Blasticidin S as a selection agent for cells carrying plasmids conferring blasticidin resistance. The resistance is mediated by the blasticidin S deaminase genes (bsr from Bacillus cereus or BSD from Aspergillus terreus). These genes produce enzymes that catalyze the hydrolytic deamination of the cytosine moiety in blasticidin S, leading to the formation of a non-toxic deaminohydroxy derivative. This resistance mechanism is crucial in various studies involving genetic manipulations and selections in cell biology and biochemical research.
- Application: Blasticidin S has been: studies to have fungicidal properties and prevents rice blast disease.[1]used as a selection agent for transformed cells that contain the resistance genes bls, bsr, or bsd. Blasticidin S has been used to select HEK293-T cells with TLR-2 constructs and HEK-D5 cells.[2][3]used to study protein synthesis at the level of peptide bond formation.
- Biochem/physiol Actions: Mode of Action: It inhibits protein synthesis in bacteria and eukaryotes.[4] Blasticidin S inhibits hydrolysis of peptidyl-tRNA induced by release factors. It enhances the binding of tRNA to the large subunit of ribosomes and to an extent inhibits peptide bond formation.[4][5]Antimicrobial Spectrum: Active against mycobacteria, several Gram-positive and Gram-negative bacteria
- Features and Benefits: High-quality antibiotic suitable for multiple research applicationsIdeal for Cell Biology and Biochemical research
- Other Notes: For additional information on our range of Biochemicals, please complete this form.
SAFETY INFORMATION
Pictograms
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 3 Oral
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Compare Similar Items
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Quality Level: 200
sterility: 0.22 μm filtered
Assay: ≥98% (HPLC)
form: liquid
concentration: 10 mg/mL (20 mM HEPES)
color: colorless to faint yellow
antibiotic activity spectrum: fungi
Mode of action: protein synthesis | interferes
storage temp.: −20°C
SMILES string: CN(CCC(CC(=O)NC1C=CC(OC1C(=O)O)N2C=CC(=NC2=O)N)N)C(=N)N.Cl
Quality Level:
200
sterility:
0.22 μm filtered
Assay:
≥98% (HPLC)
form:
liquid
concentration:
10 mg/mL (20 mM HEPES)
color:
colorless to faint yellow
antibiotic activity spectrum:
fungi
Mode of action:
protein synthesis | interferes
storage temp.:
−20°C
SMILES string:
CN(CCC(CC(=O)NC1C=CC(OC1C(=O)O)N2C=CC(=NC2=O)N)N)C(=N)N.Cl
Quality Level: __
sterility: __
Assay: ≥98% (HPLC)
form: liquid
concentration: 25 mg/mL in DMSO
color: colorless to faint yellow
antibiotic activity spectrum: Gram-negative bacteriaGram-positive bacteriamycobacteria
Mode of action: DNA synthesis | interferesenzyme | inhibits
storage temp.: −20°C
SMILES string: __
Quality Level:
__
sterility:
__
Assay:
≥98% (HPLC)
form:
liquid
concentration:
25 mg/mL in DMSO
color:
colorless to faint yellow
antibiotic activity spectrum:
Gram-negative bacteriaGram-positive bacteriamycobacteria
Mode of action:
DNA synthesis | interferesenzyme | inhibits
storage temp.:
−20°C
SMILES string:
__
Quality Level: __
sterility: __
Assay: ≥98% (HPLC)
form: liquid
concentration: 25 mg/mL in DMSO
color: colorless to faint yellow
antibiotic activity spectrum: Gram-negative bacteriaGram-positive bacteriamycobacteria
Mode of action: DNA synthesis | interferesenzyme | inhibits
storage temp.: −20°C
SMILES string: __
Quality Level:
__
sterility:
__
Assay:
≥98% (HPLC)
form:
liquid
concentration:
25 mg/mL in DMSO
color:
colorless to faint yellow
antibiotic activity spectrum:
Gram-negative bacteriaGram-positive bacteriamycobacteria
Mode of action:
DNA synthesis | interferesenzyme | inhibits
storage temp.:
−20°C
SMILES string:
__
Quality Level: __
sterility: __
Assay: ≥90%
form: liquid
concentration: 100 mg/mL in water
color: faint yellow to dark brown
antibiotic activity spectrum: Gram-negative bacteriaGram-positive bacteriamycobacterianeoplastics
Mode of action: DNA synthesis | interferesenzyme | inhibits
storage temp.: −20°C
SMILES string: __
Quality Level:
__
sterility:
__
Assay:
≥90%
form:
liquid
concentration:
100 mg/mL in water
color:
faint yellow to dark brown
antibiotic activity spectrum:
Gram-negative bacteriaGram-positive bacteriamycobacterianeoplastics
Mode of action:
DNA synthesis | interferesenzyme | inhibits
storage temp.:
−20°C
SMILES string:
__