OPTIZYME™ MspI (HpaII), Fisher BioReagents™

Description

• 5'...C^C G G...3' 3'...G G C^C...5' Supplied with: 10X OPTIZYME Buffer 4 Conditions for 100% Activity: 1X OPTIZYME Buffer 4: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/ml BSA Incubate at 37°C Enzyme Activity in OPTIZYME buffers: Buffer 1: 50 - 100% Buffer 2: 50 - 100% Buffer 3: 0 - 20% Buffer 4: 100% Buffer 5: 0 - 20% Storage Buffer: 10mM potassium phosphate (pH 7.5 at 25°C), 200mM NaCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol Ligation and Re-cleavage: More than 95% of the DNA fragments can be ligated and re-cut after 50-fold over-digestion with MspI
• Note: When the external C in the sequence: CCGG is methylated, both MspI and HpaII cannot cleave
• MspI is an isoschizomer of HpaII
• However, unlike HpaII, MspI can cleave the sequence when the internal C residue is methylated
• Compatible Ends: Bsp119I, Bsu15I, Hin1I, Hin6I, MaeII, NarI, Psp1406I, SsiI, TaqI, XmiI Methylation Effects: Dam: Never overlaps - no effect Dcm: Never overlaps - no effect CpG: Completely overlaps - no effect CCGG (No effect on cleavage) EcoKI: Never overlaps - no effect EcoBI: Never overlaps - no effect