OPTIZYME™ SpeI (BcuI), Fisher BioReagents™

Description

• 5'...A^C T A G T...3' 3'...T G A T C^A...5' Supplied with: 10X OPTIZYME Buffer 4 Conditions for 100% Activity: 1X OPTIZYME Buffer 4: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/ml BSA Incubate at 37°C Enzyme Activity in OPTIZYME buffers: Buffer 1: 50 - 100% Buffer 2: 50 - 100% Buffer 3: 0 - 20% Buffer 4: 100% Buffer 5: 20 - 50% Storage Buffer: 10mM Tris-HCl (pH 7.5 at 25°C), 300mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol Ligation and Re-cleavage: More than 80% of the DNA fragments can be ligated and more than 90% of these can be re-cut after 50-fold over-digestion with SpeI
• Digestion of Agarose-embedded DNA: A minimum of 10 units of SpeI is required for the complete digestion of 1μg of agarose-embedded Adenovirus-2 DNA in 16 hours
• Note: Assayed using pUC19 DNA with insert, containing BcuI recognition site
• Compatible Ends: XmaJI, Eco130I, NheI, XbaI Methylation Effects: Dam: Never overlaps - no effect Dcm: Never overlaps - no effect CpG: Never overlaps - no effect EcoKI: May overlap - effect not determined EcoBI: May overlap - effect not determined