OPTIZYME™ NotI, Fisher BioReagents™

Description

• 5'...G C^G G C C G C...3' 3'...C G C C G G^C G...5' Conditions for 100% Activity 1X OPTIZYME Buffer 3: 50mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl 2 , 100mM NaCl and 0.1mg/ml BSA
• Incubate at 37°C
• Supplied with: 10X OPTIZYME Buffer 3 Enzyme Activity in OPTIZYME buffers Buffer 1: 0 - 20% Buffer 2: 20 - 50% Buffer 3: 100% Buffer 4: 0 - 20% Buffer 5: 20 - 50% Storage Buffer: 20mM Tris-HCl (pH 7.8 at 25°C), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 0.02% Triton X-100, 0.2mg/ml BSA and 50% (v/v) glycerol
• Ligation and Recleavage: More than 95% of DNA fragments can be ligated and recut after a 50-fold overdigestion with NotI
• Digestion of Agarose-embedded DNA: A minimum of 5 units of NotI is required for complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours
• Compatible Ends: Bsp120I, CfrI, Eco52I
• Methylation Effects: Dam: never overlaps - no effect
• Dcm: never overlaps - no effect
• CpG: completely overlaps - blocked
• GCGGCCGC (Cleavage blocked) EcoKI: never overlaps - no effect
• EcoBI: never overlaps - no effect.