BP8068-1
OPTIZYME™ Csp6I (CviQI), Fisher BioReagents™
Manufacturer: Fischer Scientific
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Concentration
10 U/μL
Incubator Temperature
37°C
For Use With (Application)
>95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with Csp6I
Content And Storage
Keep container tightly closed
Cut Site
G.TAC
Form
Liquid
Components
25 to 50% Water, >50% Glycerin
pH
7.4
Storage Buffer
10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
Quantity
1500 U
Product Type
Csp6 I
Related Products
Description
- 5'...G^T A C...3' 3'...C A T^G...5' Supplied with: 10X OPTIZYME Buffer 1 Conditions for 100% Activity: 1X OPTIZYME Buffer 1: 10mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl) 2 and 0.1mg/ml BSA Incubate at 37°C Enzyme Activity in OPTIZYME buffers: Buffer 1: 100% Buffer 2: 50 - 100% Buffer 3: 0 - 20% Buffer 4: 50 - 100% Buffer 5: 0 - 20% Storage Buffer: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol Ligation and Re-cleavage: More than 95% of the DNA fragments can be ligated and re-cut after 50-fold over-digestion with Csp6I
- Compatible Ends: FspBI, NdeI, Tru1I, VspI Methylation Effects: Dam: never overlaps - no effect Dcm: never overlaps - no effect CpG: may overlap - no effect 5'...m5C GTAm5C G...3' 3'..
- Gm5CAT Gm5C...5' (No effect on cleavage) EcoKI: never overlaps - no effect EcoBI: never overlaps - no effect
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Concentration: 10 U/μL
Incubator Temperature: 37°C
For Use With (Application): >95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with Csp6I
Content And Storage: Keep container tightly closed
Cut Site: G.TAC
Form: Liquid
Components: 25 to 50% Water, >50% Glycerin
pH: 7.4
Storage Buffer: 10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
Quantity: 1500 U
Product Type: Csp6 I
Concentration:
10 U/μL
Incubator Temperature:
37°C
For Use With (Application):
>95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with Csp6I
Content And Storage:
Keep container tightly closed
Cut Site:
G.TAC
Form:
Liquid
Components:
25 to 50% Water, >50% Glycerin
pH:
7.4
Storage Buffer:
10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
Quantity:
1500 U
Product Type:
Csp6 I
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