BP8006-1
OPTIZYME™ HindIII, Fisher BioReagents™
Manufacturer: Fischer Scientific
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Concentration
10 U/μL
Incubator Temperature
37°C
Storage Buffer
10mM Tris HCl (pH 7.5 at 25°C), 250mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
Quantity
7500 U
Product Type
HindIII
Components
25 to 50% Water, >50% Glycerin, 1 to 2.5% Potassium Chloride
For Use With (Application)
>95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with HaeIII
Content And Storage
Keep container tightly closed
Cut Site
A.AGCTT
Form
Liquid
Related Products
Description
- 5'...A^A G C T T...3' 3'...T T C G A^A...5' Supplied with: 10X OPTIZYME Buffer 5 Conditions for 100% Activity: 1X OPTIZYME Buffer 5: 10mM Tris-HCl (pH 8.5 at 37°C), 10mM MgCl 2 , 100mM KCl and 0.1mg/ml BSA Incubate at 37°C Enzyme Activity in OPTIZYME buffers: Buffer 1: 0 - 20% Buffer 2: 20 - 50% Buffer 3: 0 - 20% Buffer 4: 50 - 100% Buffer 5: 100% Storage Buffer: 10mM Tris-HCl (pH 7.5 at 25°C), 250mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol Ligation and Re-cleavage: More than 95% of the DNA fragments can be ligated and recut after a 50-fold over-digestion with HindIII
- Digestion of Agarose-embedded DNA: A minimum 5 units of HindIII is required for the complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours
- Methylation Effects: Dam: Never overlaps - no effect Dcm: Never overlaps - no effect CpG: Never overlaps - no effect EcoKI: Never overlaps - no effect EcoBI: May overlap - cleavage impaired 5'...TGm6AAGCTT(N) 3 TGCT...3' 3'...AC TTCGAA(N) 3 m6ACGA...5' (Cleavage is impaired)
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Concentration: 10 U/μL
Incubator Temperature: 37°C
Storage Buffer: 10mM Tris HCl (pH 7.5 at 25°C), 250mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
Quantity: 7500 U
Product Type: HindIII
Components: 25 to 50% Water, >50% Glycerin, 1 to 2.5% Potassium Chloride
For Use With (Application): >95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with HaeIII
Content And Storage: Keep container tightly closed
Cut Site: A.AGCTT
Form: Liquid
Concentration:
10 U/μL
Incubator Temperature:
37°C
Storage Buffer:
10mM Tris HCl (pH 7.5 at 25°C), 250mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
Quantity:
7500 U
Product Type:
HindIII
Components:
25 to 50% Water, >50% Glycerin, 1 to 2.5% Potassium Chloride
For Use With (Application):
>95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with HaeIII
Content And Storage:
Keep container tightly closed
Cut Site:
A.AGCTT
Form:
Liquid
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