BP8003-5
OPTIZYME™ EcoRI, Fisher BioReagents™
Manufacturer: Fischer Scientific
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Concentration
10 U/μL
Incubator Temperature
37°C
For Use With (Application)
>90% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with EcoRI
Content And Storage
Keep container tightly closed
Cut Site
G.AATTC
Form
Liquid
Components
25 to 50% Water, >50% Glycerin, 2.5 to10% Sodium Chloride
pH
7.4
Storage Buffer
10mM Potassium Phosphate (pH 7.4 at 25°C), 300mM NaCl, 1mM EDTA, 1mM DTT, 0.2mg/mL BSA, 0.15% Triton™ X-100, 50% Glycerol
Quantity
15,000 U
Product Type
EcoRI
Related Products
Description
- 5'...G^A A T T C...3' 3'...C T T A A^G...5' Conditions for 100% Activity: 1X OPTIZYME Buffer EcoRI: 50mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2, 100mM NaCl, 0.02% Triton X-100 and 0.1mg/ml BSA
- Incubate at 37°C
- Supplied with: 10X OPTIZYME EcoRI Buffer Enzyme Activity in OPTIZYME buffers: Buffer 1: 0 - 20% Buffer 2: No reaction Buffer 3: 100% Buffer 4: No reaction Buffer 5: 100%™ ™star activity occurs at greater than 5-fold overdigestion (5 units X 1 hour) Storage Buffer: 10mM potassium phosphate (pH 7.4 at 25°C), 300mM NaCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 0.15% Triton X-100 and 50% (v/v) glycerol
- Ligation and Recleavage: More than 95% of DNA fragments can be ligated and recut after a 50-fold overdigestion with EcoRI
- Digestion of Agarose-embedded DNA: A minimum of 5 units of EcoRI is required for complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours
- Note: Low salt concentration, large excess of the enzyme, pH>8.0, or the replacement of mg 2+ by Mn 2+ may result in star activity
- Compatible Ends: XapI, MunI, TasI
- Methylation Effects: Dam: never overlaps - no effect
- Dcm: never overlaps - no effect
- CpG: may overlap - cleavage impaired
- 5'...m5C GAATTm5C G...3' 3'..
- Gm5CTTAA Gm5...5' EcoKI: never overlaps - no effect
- EcoBI: may overlap - no effect
- 5'...TGm6AATTC(N) 4 TGCT...3' 3'...AC TTAAG(N) 4 m6ACGA...5'
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Concentration: 10 U/μL
Incubator Temperature: 37°C
For Use With (Application): >90% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with EcoRI
Content And Storage: Keep container tightly closed
Cut Site: G.AATTC
Form: Liquid
Components: 25 to 50% Water, >50% Glycerin, 2.5 to10% Sodium Chloride
pH: 7.4
Storage Buffer: 10mM Potassium Phosphate (pH 7.4 at 25°C), 300mM NaCl, 1mM EDTA, 1mM DTT, 0.2mg/mL BSA, 0.15% Triton™ X-100, 50% Glycerol
Quantity: 15,000 U
Product Type: EcoRI
Concentration:
10 U/μL
Incubator Temperature:
37°C
For Use With (Application):
>90% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with EcoRI
Content And Storage:
Keep container tightly closed
Cut Site:
G.AATTC
Form:
Liquid
Components:
25 to 50% Water, >50% Glycerin, 2.5 to10% Sodium Chloride
pH:
7.4
Storage Buffer:
10mM Potassium Phosphate (pH 7.4 at 25°C), 300mM NaCl, 1mM EDTA, 1mM DTT, 0.2mg/mL BSA, 0.15% Triton™ X-100, 50% Glycerol
Quantity:
15,000 U
Product Type:
EcoRI
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