OPTIZYME™ DdeI (HpyF31), Fisher BioReagents™

Description

• 5'...C^T N A G...3' 3'...G A N T^C...5' Supplied with: 10X OPTIZYME Buffer 4 Conditions for 100% Activity: 1X OPTIZYME Buffer 4: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/ml BSA Incubate at 37°C Enzyme Activity in OPTIZYME buffers: Buffer 1: 20 - 50% Buffer 2: 20 - 50% Buffer 3: 20 - 50% Buffer 4: 100% Buffer 5: 20 - 50% Storage Buffer: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycero l Ligation and Re-cleavage: More than 95% of the DNA fragments can be ligated and re-cut after 50-fold over-digesiton with DdeI
• Compatible Ends: BbvCI, Bpu10I, Bpu1102I, Eco81I Methylation Effects: Dam: Never overlaps - no effect Dcm: Never overlaps - no effect CpG: Never overlaps - no effect EcoKI: Never overlaps - no effect EcoBI: May overlap - effect not determined