BP8060-5

OPTIZYME™ DdeI (HpyF31), Fisher BioReagents™

Manufacturer: Fischer Scientific

Select a Size

Pack Size SKU Availability Price
Each of 1 BP8060-5-Each-of-1 In Stock ₹ 23,400.66

BP8060-5 - Each of 1

₹ 23,400.66

In Stock

Quantity

1

Base Price: ₹ 23,400.66

GST (18%): ₹ 4,212.119

Total Price: ₹ 27,612.779

Concentration

10 U/μL

Incubator Temperature

37°C

For Use With (Application)

>95% of DNA fragments can be ligated and re-cut after 50-fold over-digesiton with DdeI

Content And Storage

Keep container tightly closed

Cut Site

C.TNAG

Form

Liquid

Components

25 to 50% Water, >50% Glycerin

pH

7.4

Storage Buffer

10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol

Quantity

1000 U

Product Type

DdeI (HpyF31)

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Description

  • 5'...C^T N A G...3' 3'...G A N T^C...5' Supplied with: 10X OPTIZYME Buffer 4 Conditions for 100% Activity: 1X OPTIZYME Buffer 4: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/ml BSA Incubate at 37°C Enzyme Activity in OPTIZYME buffers: Buffer 1: 20 - 50% Buffer 2: 20 - 50% Buffer 3: 20 - 50% Buffer 4: 100% Buffer 5: 20 - 50% Storage Buffer: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycero l Ligation and Re-cleavage: More than 95% of the DNA fragments can be ligated and re-cut after 50-fold over-digesiton with DdeI
  • Compatible Ends: BbvCI, Bpu10I, Bpu1102I, Eco81I Methylation Effects: Dam: Never overlaps - no effect Dcm: Never overlaps - no effect CpG: Never overlaps - no effect EcoKI: Never overlaps - no effect EcoBI: May overlap - effect not determined

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Concentration:
10 U/μL

Incubator Temperature:
37°C

For Use With (Application):
>95% of DNA fragments can be ligated and re-cut after 50-fold over-digesiton with DdeI

Content And Storage:
Keep container tightly closed

Cut Site:
C.TNAG

Form:
Liquid

Components:
25 to 50% Water, >50% Glycerin

pH:
7.4

Storage Buffer:
10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol

Quantity:
1000 U

Product Type:
DdeI (HpyF31)

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