BP8005-5
OPTIZYME™ BamHI, Fisher BioReagents™
Manufacturer: Fischer Scientific
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Concentration
10 U/μL
Incubator Temperature
37°C
For Use With (Application)
>95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with BamHI
Content And Storage
Keep container tightly closed
Cut Site
G.GATCC
Form
Liquid
Components
25 to 50% Water, >50% Glycerin, 1 to 2.5% Sodium Chloride
pH
7.4
Storage Buffer
10mM Tris HCl (pH 7.4 at 25°C), 200mM NaCl, 1mM DTT, 0.1mM EDTA, 0.15% Triton™ X-100, 0.2mg/mL BSA, 50% Glycerol
Quantity
12,500 U
Product Type
BamHI
Related Products
Description
- 5'...G^G A T C C...3' 3'...C C T A G^G...5' Conditions for 100% Activity: 1X OPTIZYME Buffer BamHI: 10mM Tris-HCl (pH 8.0 at 37°C), 5mM MgCl 2 , 100mM KCl, 0.02% Triton X-100 and 0.1mg/ml BSA
- Incubate at 37°C Supplied with: 10X OPTIZYME BamHI Buffer Enzyme Activity in OPTIZYME buffers: Buffer 1: 20 - 50%™ Buffer 2: 100% Buffer 3: 20 - 50% Buffer 4: 100%™ Buffer 5: 50 - 100%™ ™star activity occurs at greater than 5-fold overdigestion (5 units × 1 hour) Storage Buffer: 10mM Tris-HCl (pH 7.4 at 25°C), 200mM NaCl, 1mM DTT, 0.1mM EDTA, 0.15% Triton X-100, 0.2mg/ml BSA and 50% (v/v) glycerol
- Ligation and Recleavage: More than 95% of DNA fragments can be ligated and recut after a 50-fold overdigestion with BamHI
- Digestion of Agarose-embedded DNA: A minimum of 5 units of BamHI is required for the complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours
- Note: Low salt, high glycerol (>5%) concentrations, pH >8.0 or a large excess of enzyme may result in star activity
- Compatible Ends: BclI, BglII, Bsp143I, MboI, PsuI
- Methylation Effects: Dam: completely overlaps - no effect
- Dcm: may overlap - no effect
- CpG: may overlap - no effect
- EcoKI: never overlaps - no effect
- EcoBI: never overlaps - no effect.
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Concentration: 10 U/μL
Incubator Temperature: 37°C
For Use With (Application): >95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with BamHI
Content And Storage: Keep container tightly closed
Cut Site: G.GATCC
Form: Liquid
Components: 25 to 50% Water, >50% Glycerin, 1 to 2.5% Sodium Chloride
pH: 7.4
Storage Buffer: 10mM Tris HCl (pH 7.4 at 25°C), 200mM NaCl, 1mM DTT, 0.1mM EDTA, 0.15% Triton™ X-100, 0.2mg/mL BSA, 50% Glycerol
Quantity: 12,500 U
Product Type: BamHI
Concentration:
10 U/μL
Incubator Temperature:
37°C
For Use With (Application):
>95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with BamHI
Content And Storage:
Keep container tightly closed
Cut Site:
G.GATCC
Form:
Liquid
Components:
25 to 50% Water, >50% Glycerin, 1 to 2.5% Sodium Chloride
pH:
7.4
Storage Buffer:
10mM Tris HCl (pH 7.4 at 25°C), 200mM NaCl, 1mM DTT, 0.1mM EDTA, 0.15% Triton™ X-100, 0.2mg/mL BSA, 50% Glycerol
Quantity:
12,500 U
Product Type:
BamHI
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