BP8000-1
OPTIZYME™ RsaI, Fisher BioReagents™
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | BP8000-1-Each-of-1 | In Stock | ₹ 26,438.04 |
BP8000-1 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 26,438.04
GST (18%): ₹ 4,758.847
Total Price: ₹ 31,196.887
Concentration
10 U/μL
Incubator Temperature
37°C
For Use With (Application)
>90% of DNA fragments can be ligated and re-cut after 10-fold over-digestion with Rsa I
Content And Storage
Keep container tightly closed
Cut Site
GT.AC
Form
Liquid
Components
>50% Glycerin, 25 to 50% Water
pH
7.4
Storage Buffer
10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
Quantity
1000 U
Product Type
RsaI
Related Products
Description
- 5'...G T^A C...3' 3'...C A^T G...5' Supplied with: 10X OPTIZYME Buffer 4 Conditions for 100% Activity: 1X OPTIZYME Buffer 4: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/ml BSA Incubate at 37°C Enzyme Activity in OPTIZYME buffers: Buffer 1: 50 - 100% Buffer 2: 20 - 50% Buffer 3: 0 - 20% Buffer 4: 100% Buffer 5: 0 - 20% Storage Buffer: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol Ligation and Re-cleavage: More than 95% of the DNA fragments can be ligated and re-cut after a 50-fold over-digestion with RsaI Methylation Effects: Dam: Never overlaps - no effect Dcm: Never overlaps - no effect CpG: May overlap - cleavage impaired 5'...GTAm5C G...3' 3'...CAT Gm5C...5' (Cleavage is impaired) 5'...m5C GTAm5C G...3' 3'..
- Gm5CAT Gm5C...5' (Cleavage is blocked) EcoKI: Never overlaps - no effect EcoBI: Never overlaps - no effect
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Concentration: 10 U/μL
Incubator Temperature: 37°C
For Use With (Application): >90% of DNA fragments can be ligated and re-cut after 10-fold over-digestion with Rsa I
Content And Storage: Keep container tightly closed
Cut Site: GT.AC
Form: Liquid
Components: >50% Glycerin, 25 to 50% Water
pH: 7.4
Storage Buffer: 10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
Quantity: 1000 U
Product Type: RsaI
Concentration:
10 U/μL
Incubator Temperature:
37°C
For Use With (Application):
>90% of DNA fragments can be ligated and re-cut after 10-fold over-digestion with Rsa I
Content And Storage:
Keep container tightly closed
Cut Site:
GT.AC
Form:
Liquid
Components:
>50% Glycerin, 25 to 50% Water
pH:
7.4
Storage Buffer:
10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
Quantity:
1000 U
Product Type:
RsaI
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