OPTIZYME™ XbaI, Fisher BioReagents™

Description

• 5'...T^C T A G A...3' 3'...A G A T C^T...5' Supplied with: 10X OPTIZYME Buffer 4 Conditions for 100% Activity 1X OPTIZYME Buffer 4: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/ml BSA Incubate at 37°C Enzyme Activity in OPTIZYME buffers: Buffer 1: 50 - 100% Buffer 2: 50 - 100% Buffer 3: 20 - 50% Buffer 4: 100% Buffer 5: 0 - 20% Storage Buffer: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 7mM 2-mercaptoethanol, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol Ligation and Re-cleavage: More than 95% of the DNA fragments can be ligated and re-cut after a 50-fold over-digestion with XbaI
• Digestion of Agarose-embedded DNA: A minimum 5 units of XbaI is required for the complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours
• Notes: XbaI is blocked by overlapping dam methylation
• To avoid dam methylation, use a dam-, dcm- strain
• Compatible Ends: BcuI, Eco130I, NheI, XmaJI Methylation Effects: Dam: May overlap - blocked 5'...TCTAGm6A TC...3' 3'...AGATC Tm6AG...5' (Cleavage is blocked) Dcm: Never overlaps - no effect CpG: Never overlaps - no effect EcoKI: Never overlaps - no effect EcoBI: Never overlaps - no effect