Thermo Scientific™ Cronobacter sakazakii Isolation Agar (ISO) (Dehydrated)

Description

• Detect and enumerate Cronobacter sakazakii in milk and milk samples as described in ISO TS/22964:2006 with Thermo Scientific™ Oxoid™ Cronobacter sakazakii Isolation Agar, when used in conjunction with Modified Lauryl Sulphate Broth (CM1133 and SR0247E)
• A chromogenic substrate present in the medium detects the α-glucosidase enzyme produced by Cronobacter sakazakii
• Hydrolysis of the substrate results in blue-green colonies on this medium
• Cronobacter sakazakii is a Gram-negative rod-shaped bacterium that rarely causes disease in healthy adults
• It has been implicated in outbreaks of disease in premature infants (neonates)
• Research suggests that neonates, or those infants who have other medical conditions, are more susceptible to this infection
• Most reported cases of infection are severe, including sepsis (bacteria in the blood), meningitis, or necrotizing enterocolitis (severe intestinal infection)
• Use Cronobacter sakazakii Isolation Agar for differentiation and enumeration of Cronobacter sakazakii from infant formula and other food samples
• Selective : Due to the presence of sodium desoxycholate and crystal violet the medium is selective against most Gram-positive organisms
• Easy-to-read : Cronobacter sakazakii produces blue-green colonies on this medium
• Cronobacter spp
• have been isolated at low levels from powdered infant formulae, and the organisms’ high tolerance to desiccation provides a competitive advantage in the dry environments of milk powder factories, increasing the risk of product contamination 1
• In response to concerns over this pathogen, a collaborative technical specification for the detection of Cronobacter in milk and milk products was jointly issued by ISO and IDF, as ISO/TS 22964:2006 2
• This recommended pre-enrichment in BPW, selective enrichment in mLST/vancomycin broth and plating on Enterobacter sakazakii Isolation Agar
• Positive colonies are confirmed on the basis of sugar utilisation, decarboxylase activity, as well as yellow pigmentation
• The technical specification has since been superseded and among other changes the isolation medium has been replaced with Chromogenic Cronobacter Isolation Agar (CCI) 3
• Not all products are available for sale in all territories
• Please inquire
• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand