Thermo Scientific™ Mycoplasma Agar Base (Dehydrated)

Description

• Cultivate and isolate Mycoplasma species with Thermo Scientific™ Oxoid™ Mycoplasma Agar Base (Dehydrated) when used with any satisfactory supplementary factors for the growth of Mycoplasma (PPLO)
• For the cultivation of Mycoplasma, the medium and all the supplements should be free of any toxic substances even in small amounts 1
• Mycoplasma Agar Base is free from such inhibitory or toxic substances
• It also contains a special mineral supplement which improves the growth and colony characteristics of mycoplasmas without interfering with the clarity of the medium
• Mycoplasma are a mollicute genus of bacteria that lack a cell wall around their cell membranes
• This characteristic makes them naturally resistant to many common antibiotics such as penicillin or other beta-lactam antibiotics that target cell wall synthesis
• They can be parasitic or saprotrophic
• They are mainly parasites on the surface of human and animal epithelium cells
• Mycoplasma Agar Base is formulated as a basal medium to be enriched with any satisfactory supplementary factors used for the growth of mycoplasmas
• The majority of mycoplasma require media enriched with serum for good growth
• Also antibacterial agents are necessary to prevent overgrowth of the slow-growing mycoplasmas by contaminating organisms
• Two supplements, Mycoplasma Supplement-G SR0059 and Mycoplasma Supplement-P SR0060 are developed for the improved growth of mycoplasmas
• Mycoplasma Supplement-G SR0059 is a general supplement when added to Mycoplasma Agar Base produces a complete selective medium for the propagation of sterol-requiring Mycoplasma species of the classical type
• Mycoplasma Supplement-P SR0060 is a liquid supplement based on the formulation recommended by the Mycoplasma Reference Laboratory, CPHLS, Colindale, which is used in conjunction with Mycoplasma Agar Base to form a bi-phasic medium for the isolation and preliminary identification of Mycoplasma pneumoniae
• Many species of mycoplasmas are aerobes or facultative anaerobes but some prefer micro-aerophilic conditions with the addition of carbon dioxide, or strict anaerobiosis
• Pathogenic strains grow best at 35°C while saprophytic strains often grow between 22°C and 30°C, T-strains have an optimal temperature of 36°C
• Not all products are available for sale in all territories
• Please inquire
• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.