Isolate and differentiate Salmonella species from clinical specimens and other materials using Thermo Scientific™ Remel™ Bismuth Sulfite Agar (Dehydrated)
In 1927, Wilson and Blair introduced Bismuth Sulfite Agar that selectively isolated typhoid and paratyphoid groups of bacteria from stool specimens 1
This medium has been recommended by the United States Pharmacopeia (USP) for the use in microbial limit testing 2 , the Food and Drug Administration (FDA) and American Public Health Association (APHA) for isolation of Salmonella and other pathogens from foods and dairy products 3-6
The medium contains beef extract and meat peptone which supply amino acids, peptides, carbohydrates, vitamins, and nitrogenous compounds required for the growth of microorganisms
Dextrose provides a ready source of energy
Ferrous sulphate is an indicator of hydrogen sulfide production
Bismuth is a heavy metal that has inhibitory properties for certain microorganisms
Salmonella species produce hydrogen sulfide which reacts with ferrous sulfate and forms a metallic precipitate (black or green coloration)
Selective media - Brilliant green dye is a selective agent Easy-to-read: The characteristic black and green coloration of Salmonella species comes from a metallic precipitate that forms when hydrogen sulphide, produced by Salmonella species from sulphur compounds in the medium, reacts with ferrous sulfate
Recommended by the United States Pharmacopeia (USP) for the use in microbial limit testing 2 , the Food and Drug Administration (FDA) and American Public Health Association (APHA) for isolation of Salmonella and other pathogens from foods and dairy products 3-6 .