Thermo Scientific™ Remel™ TCBS Agar

Description

• Selectively isolate Vibrio cholerae and other enteropathogenic Vibrio spp
• from clinical and non-clinical specimens using Thermo Scientific™ Remel™ TCBS Agar (Dehydrated)
• In 1963, Kobayashi 1 et al
• modified Thiosulfate-Citrate-Bile Salts-Sucrose Agar (TCBS) developed by Nakanishi ,2
• It was developed for selective isolation of vibrios which cause cholera, diarrhea, and food poisoning
• In 1982, West et al
• reported that TCBS Agar could be used for recovery of certain new pathogens such as Vibrio fluvialis and Vibrio vulnificus 3
• Vibrio cholerae is a gram-negative bacterium that causes cholera outbreaks and epidemics
• Other Vibrio species such as V
• parahaemolyticus , V
• vulnificus , V
• mimicus are well known human pathogens
• TCBS Agar has a high pH that enhances the recovery of V
• cholerae and supresses the growth of other intestinal microorganisms
• Coliforms, Proteus , Pseudomonas , and Aeromonas are partially inhibited in this medium
• Based on their ability to ferment sucrose, Vibrio spp
• produce yellow or green colonies in this medium
• Selectively isolate Vibrio cholerae and other enteropathogenic Vibrio spp
• from clinical and non-clinical specimens using TCBS Agar
• Recommended by the World Health Organization (WHO) for isolation of Vibrio cholerae 4 Recommended by AOAC International (AOAC) 5 Selective medium - Oxgall contains a mixture of bile salts which inhibits gram-positive organisms The medium contains casein and meat peptones that supply nutrients necessary for bacterial growth
• Yeast extract is a growth enhancer
• Oxgall inhibits gram-positive organisms
• Sodium thiosulfate along with ferric citrate detects the production of hydrogen sulfide
• Sucrose serves as a source of carbohydrate which is fermented by Vibrio spp
• The pH indicator system is made up by bromthymol blue and thymol blue.