Thermo Scientific™ Mannitol Egg Yolk Polymyxin Agar

Description

• Cultivate and enumerate Bacillus cereus in foods using Thermo Scientific™ Remel™ Mannitol Egg Yolk Polymyxin Agar
• It relies on the selective inhibitory component polymyxin and two indicator systems, mannitol and phenol red and egg yolk
• Thus, the growth of many unwanted organisms is suppressed
• It is recommended by the Association of Official Analytical Chemists (AOAC) and the American Public Health Association (APHA)
• 1-3 Food Poisoning by Bacillus cereus may occur when foods have been prepared and held without adequate refrigeration before being served
• B
• cereus is an adequate spore former which is commonly found in vegetables, many raw and processed foods, meat, cereals, pasteurized fresh milk and powdered milk
• This organism causes gastrointestinal illness 4
• Use Mannitol Egg Yolk Polymyxin Agar for cultivation and enumeration of Bacillus cereus in foods
• Easy-to-differentiate : Acid produced in the fermentation of mannitol by Bacillus spp other that B
• cereus causes the phenol red indicator to change the colony color from pink to yellow
• Selective : Polymyxin B is a selective agent which inhibits many gram-negative bacilli and gram-positive cocci
• Recommended by AOAC and APHA : Recommended by the Association of Official Analytical Chemists (AOAC) and the American Public Health Association (APHA)
• 1-3 Ready - to-use : Convenience of prepared media
• This medium contains beef extract and peptones that supply nitrogen, amino acids, and peptides necessary for bacterial growth
• Mannitol is the carbon source
• Acid produced in the fermentation of mannitol by Bacillus spp other that B
• cereus causes the phenol red indicator to change the colony color from pink to yellow
• B
• cereus does not ferment mannitol and produces pink colonies on MEP Agar
• Egg yolk supplies lecithin  and serves to reduce the toxic effect of organic peroxides which may accumulate in the agar
• Lecithinase, an enzyme produced by B
• cereus , metabolizes lecithin and produces an insoluble, opaque precipitation in the medium surrounding growth
• Polymyxin B is a selective agent which inhibits many gram-negative bacilli and gram-positive cocci
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• Please inquire
• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.