Thermo Scientific™ LPM Agar

Description

• Selectively isolate Listeria monocytogenes using Thermo Scientific™ Remel™ LPM Agar (Lithium chloride, Phenylethyl alcohol, Moxalactam)
• LPM Agar is a modification of McBride Agar formulated by Lee and McClain 1
• An increase in the concentration of lithium chloride and the addition of moxalactam enhances the selective properties of LPM Agar
• It is recommended by the U.S
• Department of Agriculture Food Safety Inspection Service and the American Public Health Association (APHA) for testing food and dairy samples for Listeria 2 and for isolating of Listeria monocytogenes from nonsterile sites 3
• Listeria monocytogenes is a common contaminant in the food processing environment 4,5
• Outbreaks of potentially life-threatening consumer illness is mainly caused due to contaminated meat and dairy products
• Successful recovery of Listeria from food and environmental sources depends on the ability to promote the growth of potentially injured cells and simultaneously inhibit the growth of non– Listeria background organisms 6
• Use LPM Agar for selective isolation of Listeria monocytogenes
• Selective – Glycine anhydride, lithium chloride, and phenylethyl alcohol inhibit the growth of gram-positive cocci and gram-negative bacilli
• Moxalactam, a broad-spectrum antibiotic suppresses the growth of background organisms such as Proteus , Pseudomonas , and Staphylococcus Recommended by the U.S
• Department of Agriculture Food Safety Inspection Service, and the American Public Health Association (APHA) for testing food and dairy samples for Listeria 2 Recommended for isolating Listeria monocytogenes from clinical specimens, especially those obtained from nonsterile sites
• 3 Ready-to-use – Convenience of prepared media
• This medium contains peptones and beef extract that provide nutrients which are necessary for bacterial growth
• Sodium chloride maintains osmotic equilibrium
• Glycine anhydride, lithium chloride, and phenylethyl alcohol act as selective agents and inhibit certain gram-positive and gram-negative organisms
• Moxalactam suppresses the growth of background organisms such as Proteus , Pseudomonas , and Staphylococcus spp
• Agar is the solidifying agent.