Thermo Scientific™ PRAS Chopped Meat Broth

Description

• Cultivate anaerobic microorganisms using Thermo Scientific™ Remel™ PRAS Chopped Meat Broth
• R.E
• Hungate developed the basis for these media, referred to as the roll tube technique
• The use of a steam sterilized emulsion of a brain tissue in water to support the growth of anaerobic bacilli was first developed by von Hibler 1
• Henry used the chopped meat broth for cultivating anaerobes and to study their saccharolytic and proteolytic properties 2
• Recommended by the Food and Drug Administration (FDA) and by the Association of Official Analytical Chemists (AOAC) 3
• Cultivate anaerobic microorganisms using Pre-reduced Anaerobically Sterilized (PRAS) Chopped Meat Broth
• PRAS media are processed in a reduced condition and remain reduced up to and after inoculation 4
• Ready-to-use – Convenience of a prepared medium
• Recommended by FDA for use in the enumeration and identification of Clostridium perfringens from food and by the AOAC for the detection of Clostridium botulinum 3
• Capacity to initiate growth of bacteria from minute inocula and maintain viability of cultures over extended periods of time
• This medium contains minimal salts, beef heart, peptone, and dextrose which provide reducing substances, amino acids and other nutrients necessary for the growth of anaerobes
• Solid meat particles create favorable growth conditions for anaerobes due to the reducing action of sulfhydryl groups from muscle protein
• Cysteine provides sulfahydryl groups which act as reducing agents to bind hydrogen ions and produce anaerobic conditions
• Vitamin K and hemin are essential for the growth of certain anaerobes, specifically Prevotella melaninogenica
• A mixed carbohydrate-starch source was developed to provide a carbon source for all anaerobes including those which do not use glucose
• When glucose or other carbohydrates are incorporated, a growth medium results which is useful in obtaining rapid growth from single colonies for chromatographic analyses, susceptibility testing, or inoculation of different media
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• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.