Differentiate enterococci from group D streptococci using Thermo Scientific™ Remel™ Tryptic Soy Broth with 6.5% NaCl
Tryptic Soy Broth (TSB) was originally developed without the addition of serum or blood 1 for susceptibility testing of pneumococci to sulfonamides
In 1973, Facklam recommended use of TSB w/6.5% NaCl in combination with the bile esculin test to differentiate nonenterococcal group D streptococci from Enterococcus spp 2
Enterococci are salt tolerant and will grow in TSB w/6.5% NaCl while nonenterococcal group D streptococci will not grow in salt broth 3
Used for differentiation between salt-tolerant and salt-intolerant species
Ready-to-use – Convenience of prepared medium Differential medium – Enterococcus spp
will grow in the presence of 6.5% NaCl while other streptococci, including group D streptococci (not enterococci) and some group B streptococci will not grow This medium contains casein and soy peptones that provide amino acids and other nitrogenous compounds necessary for the growth of microorganisms
Dextrose is a ready source of energy
Sodium chloride supplies essential electrolytes and maintains osmotic equilibrium
Addition of dipotassium phosphate maintains a stable pH
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