PQ1000101

Invitrogen™ ProQuest™ Two-Hybrid System with Gateway™ Technology

Manufacturer: Invitrogen™

Select a Size

Pack Size SKU Availability Price
Each of 1 PQ1000101-Each-of-1 In Stock ₹ 1,41,002.88

PQ1000101 - Each of 1

₹ 1,41,002.88

In Stock

Quantity

1

Base Price: ₹ 1,41,002.88

GST (18%): ₹ 25,380.518

Total Price: ₹ 1,66,383.398

Product Type

Two-Hybrid System

Content And Storage

The ProQuest™ Two-Hybrid System includes yeast expression vectors pDEST™32, pDEST™22, and pEXP-AD502 for generation of GAL4 DNA Binding Domain and GAL4 Activation Domain fusion proteins, and LR Clonase™ II Enzyme Mix, a glycerol stock of the MaV203 yeast strain, as well as positive and negative controls for the two-hybrid assay. Store kit components at -80°C. Guaranteed stable for six months when properly stored.

Expression System

Yeast

Product Line

Gateway™, ProQuest™

Reporter Gene

Beta-Gal (lacZ), HIS3, URA3

Assay

Two-Hybrid Assay

Quantity

1 kit

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Description

  • Reconstitutes a functional transcription factor that activates chromosomally integrated reporter genes where transcriptional activation is monitored by the growth of cells on selective media Allow rapid and easy generation of bait and prey constructs, and facilitate downstream application Low copy-number DBD (DNA Binding Domain) and AD (Activation Domain) vectors ( ARS/CEN ) to control over-expression and increase reproducibility Three different reporter genes ( HIS 3, URA 3, and lac Z) with independent promoter regions to rapidly weed out false positives URA 3 reporter gene allows for both positive and negative selection, enabling advanced two-hybrid techniques such as reverse two-hybrid A set of strong-to-weak Gateway-based control interactions to evaluate results and assess the strength of the interaction being tested Protein-Protein Interactions, Proteins, Expression, Isolation and Analysis, Yeast Two-Hybrid

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Invitrogen™

PQ1000101

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Product Type:
Two-Hybrid System

Content And Storage:
The ProQuest™ Two-Hybrid System includes yeast expression vectors pDEST™32, pDEST™22, and pEXP-AD502 for generation of GAL4 DNA Binding Domain and GAL4 Activation Domain fusion proteins, and LR Clonase™ II Enzyme Mix, a glycerol stock of the MaV203 yeast strain, as well as positive and negative controls for the two-hybrid assay. Store kit components at -80°C. Guaranteed stable for six months when properly stored.

Expression System:
Yeast

Product Line:
Gateway™, ProQuest™

Reporter Gene:
Beta-Gal (lacZ), HIS3, URA3

Assay:
Two-Hybrid Assay

Quantity:
1 kit

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15°C to 30°C

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Fischer Scientific

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Genomic DNA Purification Kit

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15°C to 30°C

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Content And Storage:
Contains sufficient reagents for 20 reactions• Positive Control RNA and Primers• AMV Reverse Transcriptase, 100 μL• Tfl DNA Polymerase, 100 μL• AMV/Tfl 5X reaction Buffer, 1 mL• MgSO, 1.25 mL• dNTP Mixture, 20 μL• 50 μL Positive ControlStore at –30° to –10°C

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Quantity:
20 Reactions