A57185
Applied Biosystems™ SteriSEQ™ Rapid Sterility Testing Kit
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | A57185-Each-of-1 | In Stock | ₹ 4,00,420.80 |
A57185 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 4,00,420.80
GST (18%): ₹ 72,075.744
Total Price: ₹ 4,72,496.544
Content And Storage
• SteriSEQ™ Assay Mix, 2 x 110 μL; Store at -25°C to -15°C and protect from light• qPCR Master Mix Plus, 2 x 750 μL; Store at -25°C to -15°C until first thawed, then store at 2°C to 8°C and protect from light• SteriSEQ™ Discriminatory Positive Control, 2 x 30 μL; Store at -25°C to -15°C• SteriSEQ™ DNA Dilution Buffer, 1 x 7 mL; Store at -25°C to -15°C until first thawed, then store at room temperature
Form
Liquid
Reaction Speed
Fast
Product Line
SteriSEQ™
No. of Reactions
100 Reactions
Detection Method
qPCR
PCR Method
qPCR
Label or Dye
FAM + VIC, ABY, Alexa Fluor 647
For Use With (Application)
Real Time PCR (qPCR)
Reaction Format
96-well
Related Products
Description
- The SteriSEQ Rapid Sterility Testing Kit is a TaqMan-based quantitative PCR (qPCR) kit for the detection of bacteria and fungi in complex bioproduction samples
- Designed and tested using criteria for rapid bacteria and fungi detection in biotherapeutic manufacturing cell culture lots, the kit meets or exceeds sensitivity and specificity guidance provided in European Pharmacopoeia (E.P 2.6.27) and U.S
- Pharmacopoeia (USP1071)
- The kit can detect 5–25 genome copies per reaction or the equivalent of 5–25 CFU per reaction
- Features of the SteriSEQ Rapid Sterility Testing Kit include: Fast —sample-to-answer turnaround time of less than a day; delivers results in <5 hours Specific —primers/probes designed specifically for bacteria (16S rRNA) and fungi (18S rRNA) Sensitive —detects bacterial and fungal species at 5–25 genome copies per reaction Efficient —minimizes use of sample material by simultaneously testing for bacteria and fungi, preserving precious cells for final product Accurate —discriminatory positive control helps eliminate false positives and an internal positive control helps ensure PCR reaction consistency in the samples The SteriSEQ Rapid Sterility Testing Kit uses predefined experiment templates that are included with the following versions of AccuSEQ Real‐Time PCR Software for PCR set up, operation, and post-run analysis: AccuSEQ Real‐Time PCR Software 3.2.1 or later (used with the QuantStudio 5 Real‐Time PCR System) AccuSEQ Real‐Time PCR Software 2.2.1 or later (used with the 7500 Fast Real‐Time PCR Instrument)
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Content And Storage: • SteriSEQ™ Assay Mix, 2 x 110 μL; Store at -25°C to -15°C and protect from light• qPCR Master Mix Plus, 2 x 750 μL; Store at -25°C to -15°C until first thawed, then store at 2°C to 8°C and protect from light• SteriSEQ™ Discriminatory Positive Control, 2 x 30 μL; Store at -25°C to -15°C• SteriSEQ™ DNA Dilution Buffer, 1 x 7 mL; Store at -25°C to -15°C until first thawed, then store at room temperature
Form: Liquid
Reaction Speed: Fast
Product Line: SteriSEQ™
No. of Reactions: 100 Reactions
Detection Method: qPCR
PCR Method: qPCR
Label or Dye: FAM + VIC, ABY, Alexa Fluor 647
For Use With (Application): Real Time PCR (qPCR)
Reaction Format: 96-well
Content And Storage:
• SteriSEQ™ Assay Mix, 2 x 110 μL; Store at -25°C to -15°C and protect from light• qPCR Master Mix Plus, 2 x 750 μL; Store at -25°C to -15°C until first thawed, then store at 2°C to 8°C and protect from light• SteriSEQ™ Discriminatory Positive Control, 2 x 30 μL; Store at -25°C to -15°C• SteriSEQ™ DNA Dilution Buffer, 1 x 7 mL; Store at -25°C to -15°C until first thawed, then store at room temperature
Form:
Liquid
Reaction Speed:
Fast
Product Line:
SteriSEQ™
No. of Reactions:
100 Reactions
Detection Method:
qPCR
PCR Method:
qPCR
Label or Dye:
FAM + VIC, ABY, Alexa Fluor 647
For Use With (Application):
Real Time PCR (qPCR)
Reaction Format:
96-well
Content And Storage: • SteriSEQ™ Assay Mix, 2 x 110 μL; Store at -25°C to -15°C and protect from light• qPCR Master Mix Plus, 2 x 750 μL; Store at -25°C to -15°C until first thawed, then store at 2°C to 8°C and protect from light• SteriSEQ™ Discriminatory Positive Control, 2 x 30 μL; Store at -25°C to -15°C• SteriSEQ™ DNA Dilution Buffer, 1 x 7 mL; Store at -25°C to -15°C until first thawed, then store at room temperature
Form: Liquid
Reaction Speed: Standard
Product Line: SteriSEQ™
No. of Reactions: 50 Reactions
Detection Method: qPCR
PCR Method: qPCR
Label or Dye: FAM + VIC, ABY, Alexa Fluor 647
For Use With (Application): Real Time PCR (qPCR)
Reaction Format: __
Content And Storage:
• SteriSEQ™ Assay Mix, 2 x 110 μL; Store at -25°C to -15°C and protect from light• qPCR Master Mix Plus, 2 x 750 μL; Store at -25°C to -15°C until first thawed, then store at 2°C to 8°C and protect from light• SteriSEQ™ Discriminatory Positive Control, 2 x 30 μL; Store at -25°C to -15°C• SteriSEQ™ DNA Dilution Buffer, 1 x 7 mL; Store at -25°C to -15°C until first thawed, then store at room temperature
Form:
Liquid
Reaction Speed:
Standard
Product Line:
SteriSEQ™
No. of Reactions:
50 Reactions
Detection Method:
qPCR
PCR Method:
qPCR
Label or Dye:
FAM + VIC, ABY, Alexa Fluor 647
For Use With (Application):
Real Time PCR (qPCR)
Reaction Format:
__
Content And Storage: Contains 1 vial with enough reagent to stain 120 slides, when 500 μL of 1X staining solution is used per slide.• Add 60 μL/vial of anhydrous DMSO to make a 1000X (1 mM) stock solution• Dissolve stock solution 1:1000 in cell imaging solution to make 1X (1 μM) staining solutionStore at room temperature. Can be stored to –20°C with no freeze/thaw cycles. When stored as instructed, these reagents are effective for a minimum of 6 months after receiving.
Form: __
Reaction Speed: __
Product Line: CellMask™
No. of Reactions: __
Detection Method: Fluorescence Microscopy
PCR Method: __
Label or Dye: __
For Use With (Application): __
Reaction Format: __
Content And Storage:
Contains 1 vial with enough reagent to stain 120 slides, when 500 μL of 1X staining solution is used per slide.• Add 60 μL/vial of anhydrous DMSO to make a 1000X (1 mM) stock solution• Dissolve stock solution 1:1000 in cell imaging solution to make 1X (1 μM) staining solutionStore at room temperature. Can be stored to –20°C with no freeze/thaw cycles. When stored as instructed, these reagents are effective for a minimum of 6 months after receiving.
Form:
__
Reaction Speed:
__
Product Line:
CellMask™
No. of Reactions:
__
Detection Method:
Fluorescence Microscopy
PCR Method:
__
Label or Dye:
__
For Use With (Application):
__
Reaction Format:
__
Content And Storage: Contains 1 vial with enough reagent to stain 120 slides, when 500 μL of 1X staining solution is used per slide.• Add 60 μL/vial of anhydrous DMSO to make a 1000X (1 mM) stock solution• Dissolve stock solution 1:1000 in cell imaging solution to make 1X (1 μM) staining solutionStore at room temperature. Can be stored to –20°C with no freeze/thaw cycles. When stored as instructed, these reagents are effective for a minimum of 6 months after receiving.
Form: __
Reaction Speed: __
Product Line: CellMask™
No. of Reactions: __
Detection Method: Fluorescence Microscopy
PCR Method: __
Label or Dye: __
For Use With (Application): __
Reaction Format: __
Content And Storage:
Contains 1 vial with enough reagent to stain 120 slides, when 500 μL of 1X staining solution is used per slide.• Add 60 μL/vial of anhydrous DMSO to make a 1000X (1 mM) stock solution• Dissolve stock solution 1:1000 in cell imaging solution to make 1X (1 μM) staining solutionStore at room temperature. Can be stored to –20°C with no freeze/thaw cycles. When stored as instructed, these reagents are effective for a minimum of 6 months after receiving.
Form:
__
Reaction Speed:
__
Product Line:
CellMask™
No. of Reactions:
__
Detection Method:
Fluorescence Microscopy
PCR Method:
__
Label or Dye:
__
For Use With (Application):
__
Reaction Format:
__