PI90060
Thermo Scientific™ TMTduplex™ Isotopic Label Reagent Set, 5 x 0.8 mg
Manufacturer: Thermo Scientific™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PI90060-Each-of-1 | In Stock | ₹ 92,062.56 |
PI90060 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 92,062.56
GST (18%): ₹ 16,571.261
Total Price: ₹ 1,08,633.821
Description
TMTduplex™ Isotopic Label Reagent Set, 5 x 0.8 mg
Product Line
TMTduplex™
Label or Dye
Tandem Mass Tag™ (TMT) Labels
Final Product Type
Protein
Workflow Step
Protein Labeling
For Use With (Equipment)
Mass Spectrometer
Content And Storage
Sufficient for: Five duplex (5 x 2-way) experiments• TMT0 Label Reagent, 5 x 0.8 mg• TMT6-127 Label Reagent, 5 x 0.8 mgStore at –20°C.
Starting Material
Cell Lysate
Detection Method
Mass Spectrometry
Quantity
5 x 0.8 mg (per tag)
Related Products
Description
- Tandem Mass Tag™ 2-plex (TMTduplex™) Isotopic Label Reagents comprise “light” TMTzero and “heavy” TMT6-127 label reagents while Tandem Mass Tag™ 2-plex (TMTduplex) Isobaric Label Reagents comprise “heavy” TMT2-126 and TMT2-127 Label Reagents for multiplexing
- Both sets of label reagents have the same structure that are NHS-activated for covalent, irreversible labeling of primary amine groups (—NH2)
- Tandem Mass Tag (TMT) Reagents are chemical tags that enable identification and quantitation of proteins in different samples using tandem mass spectrometry
- The amine-reactive, NHS-ester-activated compounds covalently attach to the peptide amino terminus and free amino termini of lysine residues of peptides and proteins with high efficiency, thereby labeling all peptides in each sample regardless of enzyme used for digestion
- During MS/MS analysis, each TMT tag is also fragmented to produce a unique reporter ion mass that is used for sample identification and quantitation
- Protein quantitation is accomplished by comparing the relative intensities of the two reporter ions in the MS/MS spectra
- Our TMTduplex reagents label all peptides for analysis of two samples in a single MS analysis
- Features of TMTduplex Isobaric and Isotopic Label Reagent Sets: • Powerful —concurrent MS analysis of two samples increases throughput and enables relative quantitation of two different treatment conditions (up to 5 experiments per set) • Consistent —identical reagent structure and performance among TMTzero, TMTduplex, TMTsixplex and TMT10plex reagents allows efficient transition from method development to multiplex quantitation, enabling biomarker discovery research across platforms and data sets • Robust —consistent chemistry allows efficient transition from method development to multiplex quantitation • Efficient —amine-reactive NHS-ester activated reagents ensure efficient labeling of all peptides regardless of protein sequence or proteolytic enzyme specificity • Compatible —optimized for use with high resolution Thermo Scientific Orbitrap MS/MS platforms, with data analysis fully supported by Proteome Discoverer™ 2.4 and above Applications: • Protein expression profiling of normal vs
- disease states or control vs
- treated • Measurement of two different samples concurrently in a single experiment • Quantitative analysis of proteins for which no antibodies are available • dentification and quantitation of membrane and post-translationally modified proteins • Identification and quantitation of hundreds to thousands of proteins in a single experiment TMT Reagents are provided as standalone sets or in optimized kit formats containing all necessary reagents and controls for maximum flexibility, convenience and reliability
- Tandem Mass Tag Reagents combined with the industry leading instruments and software provide integrated total system solutions for quantitative protein expression analysis.
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Description: TMTduplex™ Isotopic Label Reagent Set, 5 x 0.8 mg
Product Line: TMTduplex™
Label or Dye: Tandem Mass Tag™ (TMT) Labels
Final Product Type: Protein
Workflow Step: Protein Labeling
For Use With (Equipment): Mass Spectrometer
Content And Storage: Sufficient for: Five duplex (5 x 2-way) experiments• TMT0 Label Reagent, 5 x 0.8 mg• TMT6-127 Label Reagent, 5 x 0.8 mgStore at –20°C.
Starting Material: Cell Lysate
Detection Method: Mass Spectrometry
Quantity: 5 x 0.8 mg (per tag)
Description:
TMTduplex™ Isotopic Label Reagent Set, 5 x 0.8 mg
Product Line:
TMTduplex™
Label or Dye:
Tandem Mass Tag™ (TMT) Labels
Final Product Type:
Protein
Workflow Step:
Protein Labeling
For Use With (Equipment):
Mass Spectrometer
Content And Storage:
Sufficient for: Five duplex (5 x 2-way) experiments• TMT0 Label Reagent, 5 x 0.8 mg• TMT6-127 Label Reagent, 5 x 0.8 mgStore at –20°C.
Starting Material:
Cell Lysate
Detection Method:
Mass Spectrometry
Quantity:
5 x 0.8 mg (per tag)
Description: TMTsixplex™ Isobaric Label Reagent Set, 1 x 0.8 mg
Product Line: TMTsixplex™
Label or Dye: Tandem Mass Tag™ (TMT) Labels
Final Product Type: Protein
Workflow Step: Protein Labeling
For Use With (Equipment): Mass Spectrometer
Content And Storage: Sufficient for: One sixplex (1 x 6-way) experiment• TMT6-126 Label Reagent, 1 x 0.8 mg• TMT6-127 Label Reagent, 1 x 0.8 mg• TMT6-128 Label Reagent, 1 x 0.8 mg• TMT6-129 Label Reagent, 1 x 0.8 mg• TMT6-130 Label Reagent, 1 x 0.8 mg• TMT6-131 Label Reagent, 1 x 0.8 mgStore at –20°C.
Starting Material: Cell Lysate
Detection Method: Mass Spectrometry
Quantity: 1 x 0.8 mg (per tag)
Description:
TMTsixplex™ Isobaric Label Reagent Set, 1 x 0.8 mg
Product Line:
TMTsixplex™
Label or Dye:
Tandem Mass Tag™ (TMT) Labels
Final Product Type:
Protein
Workflow Step:
Protein Labeling
For Use With (Equipment):
Mass Spectrometer
Content And Storage:
Sufficient for: One sixplex (1 x 6-way) experiment• TMT6-126 Label Reagent, 1 x 0.8 mg• TMT6-127 Label Reagent, 1 x 0.8 mg• TMT6-128 Label Reagent, 1 x 0.8 mg• TMT6-129 Label Reagent, 1 x 0.8 mg• TMT6-130 Label Reagent, 1 x 0.8 mg• TMT6-131 Label Reagent, 1 x 0.8 mgStore at –20°C.
Starting Material:
Cell Lysate
Detection Method:
Mass Spectrometry
Quantity:
1 x 0.8 mg (per tag)
Description: TMTsixplex™ Isobaric Label Reagent Set, 2 x 0.8 mg
Product Line: TMTsixplex™
Label or Dye: Tandem Mass Tag™ (TMT) Labels
Final Product Type: Protein
Workflow Step: Protein Labeling
For Use With (Equipment): Mass Spectrometer
Content And Storage: Sufficient for: Two sixplex (2 x 6-way) experiments• TMT6-126 Label Reagent, 2 x 0.8 mg• TMT6-127 Label Reagent, 2 x 0.8 mg• TMT6-128 Label Reagent, 2 x 0.8 mg• TMT6-129 Label Reagent, 2 x 0.8 mg• TMT6-130 Label Reagent, 2 x 0.8 mg• TMT6-131 Label Reagent, 2 x 0.8 mgStore at –20°C.
Starting Material: Cell Lysate
Detection Method: Mass Spectrometry
Quantity: 2 x 0.8 mg (per tag)
Description:
TMTsixplex™ Isobaric Label Reagent Set, 2 x 0.8 mg
Product Line:
TMTsixplex™
Label or Dye:
Tandem Mass Tag™ (TMT) Labels
Final Product Type:
Protein
Workflow Step:
Protein Labeling
For Use With (Equipment):
Mass Spectrometer
Content And Storage:
Sufficient for: Two sixplex (2 x 6-way) experiments• TMT6-126 Label Reagent, 2 x 0.8 mg• TMT6-127 Label Reagent, 2 x 0.8 mg• TMT6-128 Label Reagent, 2 x 0.8 mg• TMT6-129 Label Reagent, 2 x 0.8 mg• TMT6-130 Label Reagent, 2 x 0.8 mg• TMT6-131 Label Reagent, 2 x 0.8 mgStore at –20°C.
Starting Material:
Cell Lysate
Detection Method:
Mass Spectrometry
Quantity:
2 x 0.8 mg (per tag)
Description: Pierce TMTduplex Isobaric Mass Tagging Kit
Product Line: TMTduplex™
Label or Dye: Tandem Mass Tag™ (TMT) Labels
Final Product Type: Protein
Workflow Step: Protein Labeling
For Use With (Equipment): Mass Spectrometer
Content And Storage: Sufficient For: 5 complete duplex (5 x 2-way) experiments• TMT0 Label Reagent, 5 x 0.8 mg• TMT2-126 Label Reagent, 5 x 0.8 mg• TMT2-127 Label Reagent, 5 x 0.8 mg• Dissolution Buffer (1M triethyl ammonium bicarbonate), 5 mL• Denaturing Reagent (10% SDS), 1 mL• Reducing Reagent (0.5M TCEP), 1 mL• Iodoacetamide, 12 x 9 mg• Quenching Reagent (50% hydroxylamine), 1 mL• Trypsin, 2 x 20 μg• Trypsin Storage Solution, 250 μL• Albumin, Bovine, 2.5 mgStore at -20°C
Starting Material: Cell Lysate
Detection Method: Mass Spectrometry
Quantity: 15 reactions
Description:
Pierce TMTduplex Isobaric Mass Tagging Kit
Product Line:
TMTduplex™
Label or Dye:
Tandem Mass Tag™ (TMT) Labels
Final Product Type:
Protein
Workflow Step:
Protein Labeling
For Use With (Equipment):
Mass Spectrometer
Content And Storage:
Sufficient For: 5 complete duplex (5 x 2-way) experiments• TMT0 Label Reagent, 5 x 0.8 mg• TMT2-126 Label Reagent, 5 x 0.8 mg• TMT2-127 Label Reagent, 5 x 0.8 mg• Dissolution Buffer (1M triethyl ammonium bicarbonate), 5 mL• Denaturing Reagent (10% SDS), 1 mL• Reducing Reagent (0.5M TCEP), 1 mL• Iodoacetamide, 12 x 9 mg• Quenching Reagent (50% hydroxylamine), 1 mL• Trypsin, 2 x 20 μg• Trypsin Storage Solution, 250 μL• Albumin, Bovine, 2.5 mgStore at -20°C
Starting Material:
Cell Lysate
Detection Method:
Mass Spectrometry
Quantity:
15 reactions