PI90062
Thermo Scientific™ TMTsixplex™ Isobaric Label Reagent Set
Manufacturer: Fischer Scientific
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PI90062-Each-of-1 | In Stock | ₹ 1,08,746.76 |
PI90062 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,08,746.76
GST (18%): ₹ 19,574.417
Total Price: ₹ 1,28,321.177
Description
TMTsixplex™ Isobaric Label Reagent Set, 2 x 0.8 mg
Product Line
TMTsixplex™
Label or Dye
Tandem Mass Tag™ (TMT) Labels
Final Product Type
Protein
Workflow Step
Protein Labeling
For Use With (Equipment)
Mass Spectrometer
Content And Storage
Sufficient for: Two sixplex (2 x 6-way) experiments• TMT6-126 Label Reagent, 2 x 0.8 mg• TMT6-127 Label Reagent, 2 x 0.8 mg• TMT6-128 Label Reagent, 2 x 0.8 mg• TMT6-129 Label Reagent, 2 x 0.8 mg• TMT6-130 Label Reagent, 2 x 0.8 mg• TMT6-131 Label Reagent, 2 x 0.8 mgStore at –20°C.
Starting Material
Cell Lysate
Detection Method
Mass Spectrometry
Quantity
2 x 0.8 mg (per tag)
Related Products
Description
- Tandem Mass Tag™ 6-plex (TMTsixplex™) Reagents are sets of isobaric compounds (i.e., same mass and structure, also called isotopomers) that are NHS-activated for covalent, irreversible labeling of primary amines (–NH2) groups
- Each isobaric reagent contains a different number of heavy isotopes in the mass reporter region, which results in a unique reporter mass during tandem MS/MS for sample identification and relative quantitation
- Amine-reactive Thermo Scientific TMT Isobaric Mass Tagging Kits and Reagents enable multiplex quantitation of proteins extracted from cells and tissues from up to 6 samples using tandem mass spectrometry
- Features of TMTsixplex Label Reagents and Kits: • Powerful —multiplexed MS analysis increases sample throughput and enables relative quantitation of up to six different samples derived from cells, tissues or biological fluids • Robust —consistent chemistry allows efficient transition from method development to multiplex quantitation, enabling biomarker discovery research • Efficient —amine-reactive NHS-ester activated reagents ensures greater than 95% labeling of all peptides regardless of protein sequence or proteolytic enzyme specificity • Compatible —optimized for use with high resolution Thermo Scientific Orbitrap MS/MS platforms, with data analysis fully supported by Proteome Discoverer™ 2.3 and above Applications of TMTsixplex Label Reagents and Kits: • Protein identification and quantitation from multiple samples of cells, tissue or biological fluids • Protein expression profiling of normal vs
- disease states or control vs
- treated • Quantitative analysis of proteins for which no antibodies are available • Identification and quantitation of membrane and post-translationally modified proteins • Identification and quantitation of hundreds to thousands of proteins in a single experiment Since TMT reagents share an identical structure and mass (i.e., isotopomers), labeled peptides co-elute during LC separation and are co-isolated during MS/MS analysis, resulting in fewer missing peptide identifications among samples
- During MS/MS analysis, each isobaric tag is also fragmented to produce a unique reporter ion mass that is used for sample identification and quantitation
- Protein quantitation is accomplished by comparing the relative intensities of the six reporter ions
- TMT Reagents are provided as standalone sets or in optimized kit formats containing all necessary reagents and controls for maximum flexibility, convenience, and reliability
- Tandem Mass Tag Reagents combined with high resolution Thermo Scientific Orbitrap instruments and Proteome Discoverer software provide integrated total system solutions for quantitative protein expression analysis
- For high resolution analysis of the TMT-labeled peptides, the recommended LC column for Nanospray Flex source is Acclaim PepMap 100 C18 LC Column (Cat
- No
- 164942 or 164939)
- For EASY-Spray source, the recommended LC column is EASY-Spray C18 LC Column (Cat
- No
- ES902 or ES903).
Compare Similar Items
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Description: TMTsixplex™ Isobaric Label Reagent Set, 2 x 0.8 mg
Product Line: TMTsixplex™
Label or Dye: Tandem Mass Tag™ (TMT) Labels
Final Product Type: Protein
Workflow Step: Protein Labeling
For Use With (Equipment): Mass Spectrometer
Content And Storage: Sufficient for: Two sixplex (2 x 6-way) experiments• TMT6-126 Label Reagent, 2 x 0.8 mg• TMT6-127 Label Reagent, 2 x 0.8 mg• TMT6-128 Label Reagent, 2 x 0.8 mg• TMT6-129 Label Reagent, 2 x 0.8 mg• TMT6-130 Label Reagent, 2 x 0.8 mg• TMT6-131 Label Reagent, 2 x 0.8 mgStore at –20°C.
Starting Material: Cell Lysate
Detection Method: Mass Spectrometry
Quantity: 2 x 0.8 mg (per tag)
Description:
TMTsixplex™ Isobaric Label Reagent Set, 2 x 0.8 mg
Product Line:
TMTsixplex™
Label or Dye:
Tandem Mass Tag™ (TMT) Labels
Final Product Type:
Protein
Workflow Step:
Protein Labeling
For Use With (Equipment):
Mass Spectrometer
Content And Storage:
Sufficient for: Two sixplex (2 x 6-way) experiments• TMT6-126 Label Reagent, 2 x 0.8 mg• TMT6-127 Label Reagent, 2 x 0.8 mg• TMT6-128 Label Reagent, 2 x 0.8 mg• TMT6-129 Label Reagent, 2 x 0.8 mg• TMT6-130 Label Reagent, 2 x 0.8 mg• TMT6-131 Label Reagent, 2 x 0.8 mgStore at –20°C.
Starting Material:
Cell Lysate
Detection Method:
Mass Spectrometry
Quantity:
2 x 0.8 mg (per tag)
Description: Pierce TMTduplex Isobaric Mass Tagging Kit
Product Line: TMTduplex™
Label or Dye: Tandem Mass Tag™ (TMT) Labels
Final Product Type: Protein
Workflow Step: Protein Labeling
For Use With (Equipment): Mass Spectrometer
Content And Storage: Sufficient For: 5 complete duplex (5 x 2-way) experiments• TMT0 Label Reagent, 5 x 0.8 mg• TMT2-126 Label Reagent, 5 x 0.8 mg• TMT2-127 Label Reagent, 5 x 0.8 mg• Dissolution Buffer (1M triethyl ammonium bicarbonate), 5 mL• Denaturing Reagent (10% SDS), 1 mL• Reducing Reagent (0.5M TCEP), 1 mL• Iodoacetamide, 12 x 9 mg• Quenching Reagent (50% hydroxylamine), 1 mL• Trypsin, 2 x 20 μg• Trypsin Storage Solution, 250 μL• Albumin, Bovine, 2.5 mgStore at -20°C
Starting Material: Cell Lysate
Detection Method: Mass Spectrometry
Quantity: 15 reactions
Description:
Pierce TMTduplex Isobaric Mass Tagging Kit
Product Line:
TMTduplex™
Label or Dye:
Tandem Mass Tag™ (TMT) Labels
Final Product Type:
Protein
Workflow Step:
Protein Labeling
For Use With (Equipment):
Mass Spectrometer
Content And Storage:
Sufficient For: 5 complete duplex (5 x 2-way) experiments• TMT0 Label Reagent, 5 x 0.8 mg• TMT2-126 Label Reagent, 5 x 0.8 mg• TMT2-127 Label Reagent, 5 x 0.8 mg• Dissolution Buffer (1M triethyl ammonium bicarbonate), 5 mL• Denaturing Reagent (10% SDS), 1 mL• Reducing Reagent (0.5M TCEP), 1 mL• Iodoacetamide, 12 x 9 mg• Quenching Reagent (50% hydroxylamine), 1 mL• Trypsin, 2 x 20 μg• Trypsin Storage Solution, 250 μL• Albumin, Bovine, 2.5 mgStore at -20°C
Starting Material:
Cell Lysate
Detection Method:
Mass Spectrometry
Quantity:
15 reactions
Description: TMTsixplex™ Isobaric Mass Tagging Kit
Product Line: TMTsixplex™
Label or Dye: Tandem Mass Tag™ (TMT) Labels
Final Product Type: Protein
Workflow Step: Protein Labeling
For Use With (Equipment): Mass Spectrometer
Content And Storage: Sufficient for: Five complete sixplex (5 x 6-way) experiments• TMT0 Label Reagent, 5 x 0.8 mg• TMT6-126 Label Reagent, 5 x 0.8 mg• TMT6-127 Label Reagent, 5 x 0.8 mg• TMT6-128 Label Reagent, 5 x 0.8 mg• TMT6-129 Label Reagent, 5 x 0.8 mg• TMT6-130 Label Reagent, 5 x 0.8 mg• TMT6-131 Label Reagent, 5 x 0.8 mg• Dissolution Buffer (1M triethyl ammonium bicarbonate), 5 mL• Denaturing Reagent (10% SDS), 1 mL• Reducing Reagent (0.5 M TCEP), 1 mL• Iodoacetamide, 12 x 9 mg• Quenching Reagent (50% hydroxylamine), 1 mL• Trypsin, 5 x 20 μg• Trypsin Storage Solution, 250 μL• Albumin, Bovine, 2.5 mgStore at -20°C.
Starting Material: Cell Lysate
Detection Method: Mass Spectrometry
Quantity: 35 Reactions
Description:
TMTsixplex™ Isobaric Mass Tagging Kit
Product Line:
TMTsixplex™
Label or Dye:
Tandem Mass Tag™ (TMT) Labels
Final Product Type:
Protein
Workflow Step:
Protein Labeling
For Use With (Equipment):
Mass Spectrometer
Content And Storage:
Sufficient for: Five complete sixplex (5 x 6-way) experiments• TMT0 Label Reagent, 5 x 0.8 mg• TMT6-126 Label Reagent, 5 x 0.8 mg• TMT6-127 Label Reagent, 5 x 0.8 mg• TMT6-128 Label Reagent, 5 x 0.8 mg• TMT6-129 Label Reagent, 5 x 0.8 mg• TMT6-130 Label Reagent, 5 x 0.8 mg• TMT6-131 Label Reagent, 5 x 0.8 mg• Dissolution Buffer (1M triethyl ammonium bicarbonate), 5 mL• Denaturing Reagent (10% SDS), 1 mL• Reducing Reagent (0.5 M TCEP), 1 mL• Iodoacetamide, 12 x 9 mg• Quenching Reagent (50% hydroxylamine), 1 mL• Trypsin, 5 x 20 μg• Trypsin Storage Solution, 250 μL• Albumin, Bovine, 2.5 mgStore at -20°C.
Starting Material:
Cell Lysate
Detection Method:
Mass Spectrometry
Quantity:
35 Reactions
Description: TMTsixplex™ Isobaric Label Reagent Set, 5 x 0.8 mg
Product Line: TMTsixplex™
Label or Dye: Tandem Mass Tag™ (TMT) Labels
Final Product Type: Protein
Workflow Step: Protein Labeling
For Use With (Equipment): Mass Spectrometer
Content And Storage: Sufficient for: Five sixplex (5 x 6-way) experiments• TMT6-126 Label Reagent, 5 x 0.8 mg• TMT6-127 Label Reagent, 5 x 0.8 mg• TMT6-128 Label Reagent, 5 x 0.8 mg• TMT6-129 Label Reagent, 5 x 0.8 mg• TMT6-130 Label Reagent, 5 x 0.8 mg• TMT6-131 Label Reagent, 5 x 0.8 mgStore at –20°C.
Starting Material: Cell Lysate
Detection Method: Mass Spectrometry
Quantity: 5 x 0.8 mg (per tag)
Description:
TMTsixplex™ Isobaric Label Reagent Set, 5 x 0.8 mg
Product Line:
TMTsixplex™
Label or Dye:
Tandem Mass Tag™ (TMT) Labels
Final Product Type:
Protein
Workflow Step:
Protein Labeling
For Use With (Equipment):
Mass Spectrometer
Content And Storage:
Sufficient for: Five sixplex (5 x 6-way) experiments• TMT6-126 Label Reagent, 5 x 0.8 mg• TMT6-127 Label Reagent, 5 x 0.8 mg• TMT6-128 Label Reagent, 5 x 0.8 mg• TMT6-129 Label Reagent, 5 x 0.8 mg• TMT6-130 Label Reagent, 5 x 0.8 mg• TMT6-131 Label Reagent, 5 x 0.8 mgStore at –20°C.
Starting Material:
Cell Lysate
Detection Method:
Mass Spectrometry
Quantity:
5 x 0.8 mg (per tag)