Thermo Scientific™ TMTsixplex™ Isobaric Label Reagent Set

Description

• Tandem Mass Tag™ 6-plex (TMTsixplex™) Reagents are sets of isobaric compounds (i.e., same mass and structure, also called isotopomers) that are NHS-activated for covalent, irreversible labeling of primary amines (–NH2) groups
• Each isobaric reagent contains a different number of heavy isotopes in the mass reporter region, which results in a unique reporter mass during tandem MS/MS for sample identification and relative quantitation
• Amine-reactive Thermo Scientific TMT Isobaric Mass Tagging Kits and Reagents enable multiplex quantitation of proteins extracted from cells and tissues from up to 6 samples using tandem mass spectrometry
• Features of TMTsixplex Label Reagents and Kits: • Powerful —multiplexed MS analysis increases sample throughput and enables relative quantitation of up to six different samples derived from cells, tissues or biological fluids • Robust —consistent chemistry allows efficient transition from method development to multiplex quantitation, enabling biomarker discovery research • Efficient —amine-reactive NHS-ester activated reagents ensures greater than 95% labeling of all peptides regardless of protein sequence or proteolytic enzyme specificity • Compatible —optimized for use with high resolution Thermo Scientific Orbitrap MS/MS platforms, with data analysis fully supported by Proteome Discoverer™ 2.3 and above Applications of TMTsixplex Label Reagents and Kits: • Protein identification and quantitation from multiple samples of cells, tissue or biological fluids • Protein expression profiling of normal vs
• disease states or control vs
• treated • Quantitative analysis of proteins for which no antibodies are available • Identification and quantitation of membrane and post-translationally modified proteins • Identification and quantitation of hundreds to thousands of proteins in a single experiment Since TMT reagents share an identical structure and mass (i.e., isotopomers), labeled peptides co-elute during LC separation and are co-isolated during MS/MS analysis, resulting in fewer missing peptide identifications among samples
• During MS/MS analysis, each isobaric tag is also fragmented to produce a unique reporter ion mass that is used for sample identification and quantitation
• Protein quantitation is accomplished by comparing the relative intensities of the six reporter ions
• TMT Reagents are provided as standalone sets or in optimized kit formats containing all necessary reagents and controls for maximum flexibility, convenience, and reliability
• Tandem Mass Tag Reagents combined with high resolution Thermo Scientific Orbitrap instruments and Proteome Discoverer software provide integrated total system solutions for quantitative protein expression analysis
• For high resolution analysis of the TMT-labeled peptides, the recommended LC column for Nanospray Flex source is Acclaim PepMap 100 C18 LC Column (Cat
• No
• 164942 or 164939)
• For EASY-Spray source, the recommended LC column is EASY-Spray C18 LC Column (Cat
• No
• ES902 or ES903).