PI90103

Thermo Scientific™ iodoTMTsixplex™ Isobaric Mass Tagging Kit

Manufacturer: Thermo Scientific™

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Pack Size SKU Availability Price
Pack of 1 PI90103-Pack-of-1 In Stock ₹ 2,98,604.40

PI90103 - Pack of 1

₹ 2,98,604.40

In Stock

Quantity

1

Base Price: ₹ 2,98,604.40

GST (18%): ₹ 53,748.792

Total Price: ₹ 3,52,353.192

Description

iodoTMTsixplex™ Isobaric Mass Tagging Kit

Product Line

iodoTMTsixplex™

Label or Dye

Tandem Mass Tag™ (TMT) Labels

Final Product Type

Protein

Workflow Step

Protein Labeling

For Use With (Equipment)

Mass Spectrometer

Content And Storage

• iodoTMT6-126 Label Reagent, 5 x 0.2 mg• iodoTMT6-127 Label Reagent, 5 x 0.2 mg• iodoTMT6-128 Label Reagent, 5 x 0.2 mg• iodoTMT6-129 Label Reagent, 5 x 0.2 mg• iodoTMT6-130 Label Reagent, 5 x 0.2 mg• iodoTMT6-131 Label Reagent, 5 x 0.2 mg• HES Buffer, 15 mL• Bond-Breaker TCEP Solution, 0.5 mL• DTT, No-Weigh Format, 8 x 7.7 mg• Trypsin, 5 x 20 μg• Trypsin Storage Solution, 250 μL• Albumin (bovine), 2.5 mgUpon receipt store all products at –20°C

Starting Material

Cell Lysate

Detection Method

Mass Spectrometry

Quantity

30 reactions

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Description

  • Thermo Scientific iodoTMTsixplex Isobaric Mass Tag Labeling Kit contains iodoTMT™ reagents and associated materials for the irreversible labeling of sulfhydryl groups
  • Iodoacetyl Tandem Mass Tag™ Reagents enable selective labeling of cysteine-containing peptides for multiplexed sample quantitation using LC-MS/MS analysis
  • The iodoTMT Reagents are sets of isobaric (mass and structure) isomers that are iodoacetyl-activated for covalent, irreversible labeling of sulfhydryl (—SH) groups
  • Similar to iodoacetamide, iodoTMT Reagents react specifically with reduced cysteines (Cys) in peptides and proteins and enable quantitation of the relative abundance of cysteine modifications, such as S-nitrosylation and the oxidation of disulfide bonds
  • The Thermo Scientific iodoTMTzero Label Reagent allows testing and optimization of sample preparation, labeling, fractionation and MS fragmentation for peptide identification and reporter detection without using the more costly isotope-labeled compounds
  • Features of the iodoTMT Reagents: • Specific —only reacts with sulfhydryl groups (reduced, unmodified cysteine residues) • Irreversible —labeled proteins and peptides are not susceptible to reducing agents • Flexible —options for duplex isotopic (MS) or sixplex isobaric (MS/MS) quantitation • Versatile —can be used to study cysteine modifications (e.g
  • di-sulfide bridges, S-nitrosylation) To selectively analyze the cysteine-labeled peptides, the sample may be enriched using the Thermo Scientific Immobilized Anti-TMT Antibody Resin (Cat
  • No
  • 90076) and TMT Elution Buffer (Cat
  • No
  • 90104)
  • This approach of selective thiol-labeling and affinity enrichment is similar to isotope-coded affinity tags (ICAT™ Method) but allows for affinity enrichment with a non-biological tag and higher multiplex quantitation.

Compare Similar Items

Show Difference

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Thermo Scientific™

PI90103

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Description:
iodoTMTsixplex™ Isobaric Mass Tagging Kit

Product Line:
iodoTMTsixplex™

Label or Dye:
Tandem Mass Tag™ (TMT) Labels

Final Product Type:
Protein

Workflow Step:
Protein Labeling

For Use With (Equipment):
Mass Spectrometer

Content And Storage:
• iodoTMT6-126 Label Reagent, 5 x 0.2 mg• iodoTMT6-127 Label Reagent, 5 x 0.2 mg• iodoTMT6-128 Label Reagent, 5 x 0.2 mg• iodoTMT6-129 Label Reagent, 5 x 0.2 mg• iodoTMT6-130 Label Reagent, 5 x 0.2 mg• iodoTMT6-131 Label Reagent, 5 x 0.2 mg• HES Buffer, 15 mL• Bond-Breaker TCEP Solution, 0.5 mL• DTT, No-Weigh Format, 8 x 7.7 mg• Trypsin, 5 x 20 μg• Trypsin Storage Solution, 250 μL• Albumin (bovine), 2.5 mgUpon receipt store all products at –20°C

Starting Material:
Cell Lysate

Detection Method:
Mass Spectrometry

Quantity:
30 reactions

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Fischer Scientific

PI90104

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Description:
TMT™ Elution Buffer

Product Line:
TMT™

Label or Dye:
Tandem Mass Tag™ (TMT) Labels

Final Product Type:
Peptide

Workflow Step:
Protein Labeling

For Use With (Equipment):
Mass Spectrometer

Content And Storage:
Store at 4°C.

Starting Material:
Cell Lysate

Detection Method:
Mass Spectrometry

Quantity:
20 mL

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Fischer Scientific

PI90105

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Description:
Pierce™ S-Nitrosylation Western Blot Kit

Product Line:
Pierce™

Label or Dye:
__

Final Product Type:
__

Workflow Step:
__

For Use With (Equipment):
__

Content And Storage:
Upon receipt store kit at –20°C, or store individual components as indicated above. Kit is shipped with an ice pack.

Starting Material:
__

Detection Method:
S-Nitrosylation

Quantity:
40 Reactions

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Fischer Scientific

PI90106

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Description:
HENS Buffer for Pierce™ S-Nitrosylation Western Blot Kit

Product Line:
Pierce™

Label or Dye:
__

Final Product Type:
__

Workflow Step:
__

For Use With (Equipment):
__

Content And Storage:
HENS Buffer is stored at room temperature and is shipped at ambient temperature.

Starting Material:
__

Detection Method:
S-Nitrosylation

Quantity:
100 mL