89-014-387
SKOV-3 (human adenocarcinoma) Whole cell lysate, Non-denatured; Abnova
Manufacturer: Abnova Corporation
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 89-014-387-Each-of-1 | In Stock | ₹ 20,705.52 |
89-014-387 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 20,705.52
GST (18%): ₹ 3,726.994
Total Price: ₹ 24,432.514
For Use With (Application)
Immunoprecipitation, Western Blot
Description
Whole cell lysate (non-denatured)
Preparation Method
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
Quantity
200 μg
Host Species
Human
Content And Storage
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Tissue
Ovary
Lysis Buffer
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer
In modified RIPA Lysis Buffer.
Concentration
5 mg/mL
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Description
- Western Blotting, Immunoprecipitation
Compare Similar Items
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For Use With (Application): Immunoprecipitation, Western Blot
Description: Whole cell lysate (non-denatured)
Preparation Method: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
Quantity: 200 μg
Host Species: Human
Content And Storage: Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Tissue: Ovary
Lysis Buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Quality Control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer: In modified RIPA Lysis Buffer.
Concentration: 5 mg/mL
For Use With (Application):
Immunoprecipitation, Western Blot
Description:
Whole cell lysate (non-denatured)
Preparation Method:
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
Quantity:
200 μg
Host Species:
Human
Content And Storage:
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Tissue:
Ovary
Lysis Buffer:
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Quality Control Testing:
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer:
In modified RIPA Lysis Buffer.
Concentration:
5 mg/mL
For Use With (Application): Immunoprecipitation, Western Blot
Description: Whole cell lysate (non-denatured)
Preparation Method: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
Quantity: 200 μg
Host Species: Human
Content And Storage: Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Tissue: Breast
Lysis Buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Quality Control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer: In modified RIPA Lysis Buffer.
Concentration: 5 mg/mL
For Use With (Application):
Immunoprecipitation, Western Blot
Description:
Whole cell lysate (non-denatured)
Preparation Method:
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
Quantity:
200 μg
Host Species:
Human
Content And Storage:
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Tissue:
Breast
Lysis Buffer:
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Quality Control Testing:
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer:
In modified RIPA Lysis Buffer.
Concentration:
5 mg/mL
For Use With (Application): Immunoprecipitation, Western Blot
Description: Whole cell lysate (non-denatured)
Preparation Method: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
Quantity: 200 μg
Host Species: Human
Content And Storage: Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Tissue: Connective Tissue, Fibrosarcoma
Lysis Buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Quality Control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer: In modified RIPA Lysis Buffer.
Concentration: __
For Use With (Application):
Immunoprecipitation, Western Blot
Description:
Whole cell lysate (non-denatured)
Preparation Method:
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
Quantity:
200 μg
Host Species:
Human
Content And Storage:
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Tissue:
Connective Tissue, Fibrosarcoma
Lysis Buffer:
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Quality Control Testing:
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer:
In modified RIPA Lysis Buffer.
Concentration:
__
For Use With (Application): Immunoprecipitation, Western Blot
Description: Whole cell lysate (non-denatured)
Preparation Method: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
Quantity: 200 μg
Host Species: Human
Content And Storage: Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Tissue: Colon, Colorectal Carcinoma
Lysis Buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Quality Control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer: In modified RIPA Lysis Buffer.
Concentration: 5 mg/mL
For Use With (Application):
Immunoprecipitation, Western Blot
Description:
Whole cell lysate (non-denatured)
Preparation Method:
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
Quantity:
200 μg
Host Species:
Human
Content And Storage:
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Tissue:
Colon, Colorectal Carcinoma
Lysis Buffer:
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Quality Control Testing:
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer:
In modified RIPA Lysis Buffer.
Concentration:
5 mg/mL