89-014-389
SK-BR-3 (human breast adenocarcinoma) Whole cell lysate, Non-denatured; Abnova
Manufacturer: Abnova Corporation
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 89-014-389-Each-of-1 | In Stock | ₹ 22,288.38 |
89-014-389 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 22,288.38
GST (18%): ₹ 4,011.908
Total Price: ₹ 26,300.288
For Use With (Application)
Immunoprecipitation, Western Blot
Description
Whole cell lysate (non-denatured)
Preparation Method
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
Quantity
200 μg
Host Species
Human
Content And Storage
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Tissue
Breast
Lysis Buffer
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer
In modified RIPA Lysis Buffer.
Concentration
5 mg/mL
Related Products
Description
- Tissue: Breast Host: Human Lysis buffer: Modified RIPA lysis buffer: 50mM Tris-HCl pH 7.4, 150mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF Storage buffer: In modified RIPA lysis buffer Western Blotting, Immunoprecipitation
Compare Similar Items
Show Difference
For Use With (Application): Immunoprecipitation, Western Blot
Description: Whole cell lysate (non-denatured)
Preparation Method: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
Quantity: 200 μg
Host Species: Human
Content And Storage: Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Tissue: Breast
Lysis Buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Quality Control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer: In modified RIPA Lysis Buffer.
Concentration: 5 mg/mL
For Use With (Application):
Immunoprecipitation, Western Blot
Description:
Whole cell lysate (non-denatured)
Preparation Method:
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
Quantity:
200 μg
Host Species:
Human
Content And Storage:
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Tissue:
Breast
Lysis Buffer:
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Quality Control Testing:
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer:
In modified RIPA Lysis Buffer.
Concentration:
5 mg/mL
For Use With (Application): Immunoprecipitation, Western Blot
Description: Whole cell lysate (non-denatured)
Preparation Method: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
Quantity: 200 μg
Host Species: Human
Content And Storage: Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Tissue: Connective Tissue, Fibrosarcoma
Lysis Buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Quality Control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer: In modified RIPA Lysis Buffer.
Concentration: __
For Use With (Application):
Immunoprecipitation, Western Blot
Description:
Whole cell lysate (non-denatured)
Preparation Method:
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
Quantity:
200 μg
Host Species:
Human
Content And Storage:
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Tissue:
Connective Tissue, Fibrosarcoma
Lysis Buffer:
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Quality Control Testing:
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer:
In modified RIPA Lysis Buffer.
Concentration:
__
For Use With (Application): Immunoprecipitation, Western Blot
Description: Whole cell lysate (non-denatured)
Preparation Method: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
Quantity: 200 μg
Host Species: Human
Content And Storage: Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Tissue: Colon, Colorectal Carcinoma
Lysis Buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Quality Control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer: In modified RIPA Lysis Buffer.
Concentration: 5 mg/mL
For Use With (Application):
Immunoprecipitation, Western Blot
Description:
Whole cell lysate (non-denatured)
Preparation Method:
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
Quantity:
200 μg
Host Species:
Human
Content And Storage:
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Tissue:
Colon, Colorectal Carcinoma
Lysis Buffer:
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Quality Control Testing:
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer:
In modified RIPA Lysis Buffer.
Concentration:
5 mg/mL
For Use With (Application): Western Blot
Description: __
Preparation Method: Cell Lysate was prepared by homogenization in ice cold modified RIPA Lysis Buffer with cocktail of Protease inhibitors (Sigma), cell debris was removed by centrifugation, Protein concentration was determined with Bradford assay
Quantity: 200 μg
Host Species: Marsupial
Content And Storage: -80°C
Tissue: Marsupial Kidney, Normal
Lysis Buffer: Modified RIPA Lysis Buffer: 50mM Tris HCl, pH 7.4, 150mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium Deoxycholate, 1mM PMSF
Quality Control Testing: 12.5% SDS-PAGE stained with Coomassie Blue
Storage Buffer: Modified RIPA Lysis Buffer
Concentration: 5 mg/mL
For Use With (Application):
Western Blot
Description:
__
Preparation Method:
Cell Lysate was prepared by homogenization in ice cold modified RIPA Lysis Buffer with cocktail of Protease inhibitors (Sigma), cell debris was removed by centrifugation, Protein concentration was determined with Bradford assay
Quantity:
200 μg
Host Species:
Marsupial
Content And Storage:
-80°C
Tissue:
Marsupial Kidney, Normal
Lysis Buffer:
Modified RIPA Lysis Buffer: 50mM Tris HCl, pH 7.4, 150mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium Deoxycholate, 1mM PMSF
Quality Control Testing:
12.5% SDS-PAGE stained with Coomassie Blue
Storage Buffer:
Modified RIPA Lysis Buffer
Concentration:
5 mg/mL