89-014-392

PtK2 (human kidney) Whole cell lysate, Non-denatured; Abnova

Manufacturer: Abnova Corporation

Select a Size

Pack Size SKU Availability Price
Each of 1 89-014-392-Each-of-1 In Stock ₹ 23,186.76

89-014-392 - Each of 1

₹ 23,186.76

In Stock

Quantity

1

Base Price: ₹ 23,186.76

GST (18%): ₹ 4,173.617

Total Price: ₹ 27,360.377

For Use With (Application)

Western Blot

Lysis Buffer

Modified RIPA Lysis Buffer: 50mM Tris HCl, pH 7.4, 150mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium Deoxycholate, 1mM PMSF

Quality Control Testing

12.5% SDS-PAGE stained with Coomassie Blue

Storage Buffer

Modified RIPA Lysis Buffer

Concentration

5 mg/mL

Tissue

Marsupial Kidney, Normal

Preparation Method

Cell Lysate was prepared by homogenization in ice cold modified RIPA Lysis Buffer with cocktail of Protease inhibitors (Sigma), cell debris was removed by centrifugation, Protein concentration was determined with Bradford assay

Quantity

200 μg

Host Species

Marsupial

Content And Storage

-80°C

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Description

  • Western Blotting, Immunoprecipitation

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Abnova Corporation

89-014-392

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For Use With (Application):
Western Blot

Lysis Buffer:
Modified RIPA Lysis Buffer: 50mM Tris HCl, pH 7.4, 150mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium Deoxycholate, 1mM PMSF

Quality Control Testing:
12.5% SDS-PAGE stained with Coomassie Blue

Storage Buffer:
Modified RIPA Lysis Buffer

Concentration:
5 mg/mL

Tissue:
Marsupial Kidney, Normal

Preparation Method:
Cell Lysate was prepared by homogenization in ice cold modified RIPA Lysis Buffer with cocktail of Protease inhibitors (Sigma), cell debris was removed by centrifugation, Protein concentration was determined with Bradford assay

Quantity:
200 μg

Host Species:
Marsupial

Content And Storage:
-80°C

Img

Abnova Corporation

89-014-393

--


For Use With (Application):
Western Blot

Lysis Buffer:
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.

Quality Control Testing:
12.5% SDS-PAGE Stained with Coomassie Blue.

Storage Buffer:
In modified RIPA Lysis Buffer.

Concentration:
5 mg/mL

Tissue:
Kidney, SV40-transformed

Preparation Method:
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).

Quantity:
200 μg

Host Species:
African Green Monkey

Content And Storage:
Store at -80°C. Aliquot to avoid repeated freezing and thawing.

Img

Abnova Corporation

89-014-395

--


For Use With (Application):
Immunoprecipitation, Western Blot

Lysis Buffer:
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.

Quality Control Testing:
12.5% SDS-PAGE Stained with Coomassie Blue.

Storage Buffer:
In modified RIPA Lysis Buffer.

Concentration:
__

Tissue:
Colon

Preparation Method:
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).

Quantity:
200 μg

Host Species:
Human

Content And Storage:
Store at -80°C. Aliquot to avoid repeated freezing and thawing.

Img

Abnova Corporation

89-014-396

--


For Use With (Application):
Immunoprecipitation, Western Blot

Lysis Buffer:
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.

Quality Control Testing:
12.5% SDS-PAGE Stained with Coomassie Blue.

Storage Buffer:
In modified RIPA Lysis Buffer.

Concentration:
__

Tissue:
Ovary

Preparation Method:
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).

Quantity:
200 μg

Host Species:
Hamster

Content And Storage:
Store at -80°C. Aliquot to avoid repeated freezing and thawing.