Manufacturer: Abnova Corporation
Pack Size | SKU | Availability | Price |
---|---|---|---|
Each of 1 | 89-014-393-Each-of-1 | In Stock | ₹ 23,956.80 |
89-014-393 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 23,956.80
GST (18%): ₹ 4,312.224
Total Price: ₹ 28,269.024
Kidney, SV40-transformed
Whole cell lysate (non-denatured)
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
200 μg
African Green Monkey
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Western Blot
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
12.5% SDS-PAGE Stained with Coomassie Blue.
In modified RIPA Lysis Buffer.
5 mg/mL