89-014-393

COS-7 (African green monkey kidney) Whole cell lysate, Non-denatured; Abnova

Manufacturer: Abnova Corporation

Select a Size

Pack Size SKU Availability Price
Each of 1 89-014-393-Each-of-1 In Stock ₹ 23,956.80

89-014-393 - Each of 1

₹ 23,956.80

In Stock

Quantity

1

Base Price: ₹ 23,956.80

GST (18%): ₹ 4,312.224

Total Price: ₹ 28,269.024

Tissue

Kidney, SV40-transformed

Description

Whole cell lysate (non-denatured)

Preparation Method

Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).

Quantity

200 μg

Host Species

African Green Monkey

Content And Storage

Store at -80°C. Aliquot to avoid repeated freezing and thawing.

For Use With (Application)

Western Blot

Lysis Buffer

Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.

Quality Control Testing

12.5% SDS-PAGE Stained with Coomassie Blue.

Storage Buffer

In modified RIPA Lysis Buffer.

Concentration

5 mg/mL

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Description

  • Tissue: Kidney; Sv40 transformed Host: African Green Monkey Lysis buffer: Modified RIPA lysis buffer: 50mM Tris-HCl pH 7.4, 150mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF Storage buffer: In modified RIPA lysis buffer Western Blotting, Immunoprecipitation

Compare Similar Items

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Abnova Corporation

89-014-393

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Tissue:
Kidney, SV40-transformed

Description:
Whole cell lysate (non-denatured)

Preparation Method:
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).

Quantity:
200 μg

Host Species:
African Green Monkey

Content And Storage:
Store at -80°C. Aliquot to avoid repeated freezing and thawing.

For Use With (Application):
Western Blot

Lysis Buffer:
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.

Quality Control Testing:
12.5% SDS-PAGE Stained with Coomassie Blue.

Storage Buffer:
In modified RIPA Lysis Buffer.

Concentration:
5 mg/mL

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Abnova Corporation

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Tissue:
Colon

Description:
Whole cell lysate (non-denatured)

Preparation Method:
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).

Quantity:
200 μg

Host Species:
Human

Content And Storage:
Store at -80°C. Aliquot to avoid repeated freezing and thawing.

For Use With (Application):
Immunoprecipitation, Western Blot

Lysis Buffer:
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.

Quality Control Testing:
12.5% SDS-PAGE Stained with Coomassie Blue.

Storage Buffer:
In modified RIPA Lysis Buffer.

Concentration:
__

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Tissue:
Ovary

Description:
Whole cell lysate (non-denatured)

Preparation Method:
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).

Quantity:
200 μg

Host Species:
Hamster

Content And Storage:
Store at -80°C. Aliquot to avoid repeated freezing and thawing.

For Use With (Application):
Immunoprecipitation, Western Blot

Lysis Buffer:
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.

Quality Control Testing:
12.5% SDS-PAGE Stained with Coomassie Blue.

Storage Buffer:
In modified RIPA Lysis Buffer.

Concentration:
__

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Abnova Corporation

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Tissue:
Skeletal Muscle

Description:
Whole cell lysate (non-denatured)

Preparation Method:
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).

Quantity:
200 μg

Host Species:
Mouse

Content And Storage:
Store at -80°C. Aliquot to avoid repeated freezing and thawing.

For Use With (Application):
Immunoprecipitation, Western Blot

Lysis Buffer:
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.

Quality Control Testing:
12.5% SDS-PAGE Stained with Coomassie Blue.

Storage Buffer:
In modified RIPA Lysis Buffer.

Concentration:
__