89-014-395
SW480 (Human colon adenocarcinoma) Whole cell lysate, Non-denatured; Abnova
Manufacturer: Abnova Corporation
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 89-014-395-Each-of-1 | In Stock | ₹ 19,764.36 |
89-014-395 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 19,764.36
GST (18%): ₹ 3,557.585
Total Price: ₹ 23,321.945
For Use With (Application)
Immunoprecipitation, Western Blot
Description
Whole cell lysate (non-denatured)
Preparation Method
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
Quantity
200 μg
Host Species
Human
Tissue
Colon
Lysis Buffer
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer
In modified RIPA Lysis Buffer.
Content And Storage
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Related Products
Description
- Tissue: Colon Host: Human Lysis buffer: Modified RIPA Lysis Buffer: 50mM Tris-HCl pH 7.4, 150mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF Storage buffer: In modified RIPA lysis buffer Western Blotting, Immunoprecipitation
Compare Similar Items
Show Difference
For Use With (Application): Immunoprecipitation, Western Blot
Description: Whole cell lysate (non-denatured)
Preparation Method: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
Quantity: 200 μg
Host Species: Human
Tissue: Colon
Lysis Buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Quality Control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer: In modified RIPA Lysis Buffer.
Content And Storage: Store at -80°C. Aliquot to avoid repeated freezing and thawing.
For Use With (Application):
Immunoprecipitation, Western Blot
Description:
Whole cell lysate (non-denatured)
Preparation Method:
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
Quantity:
200 μg
Host Species:
Human
Tissue:
Colon
Lysis Buffer:
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Quality Control Testing:
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer:
In modified RIPA Lysis Buffer.
Content And Storage:
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
For Use With (Application): Immunoprecipitation, Western Blot
Description: Whole cell lysate (non-denatured)
Preparation Method: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
Quantity: 200 μg
Host Species: Hamster
Tissue: Ovary
Lysis Buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Quality Control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer: In modified RIPA Lysis Buffer.
Content And Storage: Store at -80°C. Aliquot to avoid repeated freezing and thawing.
For Use With (Application):
Immunoprecipitation, Western Blot
Description:
Whole cell lysate (non-denatured)
Preparation Method:
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
Quantity:
200 μg
Host Species:
Hamster
Tissue:
Ovary
Lysis Buffer:
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Quality Control Testing:
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer:
In modified RIPA Lysis Buffer.
Content And Storage:
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
For Use With (Application): Immunoprecipitation, Western Blot
Description: Whole cell lysate (non-denatured)
Preparation Method: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
Quantity: 200 μg
Host Species: Mouse
Tissue: Skeletal Muscle
Lysis Buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Quality Control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer: In modified RIPA Lysis Buffer.
Content And Storage: Store at -80°C. Aliquot to avoid repeated freezing and thawing.
For Use With (Application):
Immunoprecipitation, Western Blot
Description:
Whole cell lysate (non-denatured)
Preparation Method:
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
Quantity:
200 μg
Host Species:
Mouse
Tissue:
Skeletal Muscle
Lysis Buffer:
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Quality Control Testing:
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer:
In modified RIPA Lysis Buffer.
Content And Storage:
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
For Use With (Application): Immunoprecipitation, Western Blot
Description: Whole cell lysate (non-denatured)
Preparation Method: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
Quantity: 200 μg
Host Species: Mouse
Tissue: Lung
Lysis Buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Quality Control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer: In modified RIPA Lysis Buffer.
Content And Storage: Store at -80°C. Aliquot to avoid repeated freezing and thawing.
For Use With (Application):
Immunoprecipitation, Western Blot
Description:
Whole cell lysate (non-denatured)
Preparation Method:
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
Quantity:
200 μg
Host Species:
Mouse
Tissue:
Lung
Lysis Buffer:
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Quality Control Testing:
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer:
In modified RIPA Lysis Buffer.
Content And Storage:
Store at -80°C. Aliquot to avoid repeated freezing and thawing.