89-014-390
HT1080 (human fibrosarcoma) Whole cell lysate, Non-denatured; Abnova
Manufacturer: Abnova Corporation
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 89-014-390-Each-of-1 | In Stock | ₹ 21,047.76 |
89-014-390 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 21,047.76
GST (18%): ₹ 3,788.597
Total Price: ₹ 24,836.357
Tissue
Connective Tissue, Fibrosarcoma
Description
Whole cell lysate (non-denatured)
Preparation Method
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
Quantity
200 μg
Host Species
Human
For Use With (Application)
Immunoprecipitation, Western Blot
Lysis Buffer
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer
In modified RIPA Lysis Buffer.
Content And Storage
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Related Products
Description
- Tissue: Connective Tissue, Fibrosarcoma Host: Human Lysis Buffer: Modified RIPA lysis buffer: 50mM Tris-HCl pH 7.4, 150mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF Storage buffer: In modified RIPA lysis buffer Western Blotting, Immunoprecipitation
Compare Similar Items
Show Difference
Tissue: Connective Tissue, Fibrosarcoma
Description: Whole cell lysate (non-denatured)
Preparation Method: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
Quantity: 200 μg
Host Species: Human
For Use With (Application): Immunoprecipitation, Western Blot
Lysis Buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Quality Control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer: In modified RIPA Lysis Buffer.
Content And Storage: Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Tissue:
Connective Tissue, Fibrosarcoma
Description:
Whole cell lysate (non-denatured)
Preparation Method:
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
Quantity:
200 μg
Host Species:
Human
For Use With (Application):
Immunoprecipitation, Western Blot
Lysis Buffer:
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Quality Control Testing:
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer:
In modified RIPA Lysis Buffer.
Content And Storage:
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Tissue: Colon, Colorectal Carcinoma
Description: Whole cell lysate (non-denatured)
Preparation Method: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
Quantity: 200 μg
Host Species: Human
For Use With (Application): Immunoprecipitation, Western Blot
Lysis Buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Quality Control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer: In modified RIPA Lysis Buffer.
Content And Storage: Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Tissue:
Colon, Colorectal Carcinoma
Description:
Whole cell lysate (non-denatured)
Preparation Method:
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
Quantity:
200 μg
Host Species:
Human
For Use With (Application):
Immunoprecipitation, Western Blot
Lysis Buffer:
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Quality Control Testing:
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer:
In modified RIPA Lysis Buffer.
Content And Storage:
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Tissue: Marsupial Kidney, Normal
Description: __
Preparation Method: Cell Lysate was prepared by homogenization in ice cold modified RIPA Lysis Buffer with cocktail of Protease inhibitors (Sigma), cell debris was removed by centrifugation, Protein concentration was determined with Bradford assay
Quantity: 200 μg
Host Species: Marsupial
For Use With (Application): Western Blot
Lysis Buffer: Modified RIPA Lysis Buffer: 50mM Tris HCl, pH 7.4, 150mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium Deoxycholate, 1mM PMSF
Quality Control Testing: 12.5% SDS-PAGE stained with Coomassie Blue
Storage Buffer: Modified RIPA Lysis Buffer
Content And Storage: -80°C
Tissue:
Marsupial Kidney, Normal
Description:
__
Preparation Method:
Cell Lysate was prepared by homogenization in ice cold modified RIPA Lysis Buffer with cocktail of Protease inhibitors (Sigma), cell debris was removed by centrifugation, Protein concentration was determined with Bradford assay
Quantity:
200 μg
Host Species:
Marsupial
For Use With (Application):
Western Blot
Lysis Buffer:
Modified RIPA Lysis Buffer: 50mM Tris HCl, pH 7.4, 150mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium Deoxycholate, 1mM PMSF
Quality Control Testing:
12.5% SDS-PAGE stained with Coomassie Blue
Storage Buffer:
Modified RIPA Lysis Buffer
Content And Storage:
-80°C
Tissue: Kidney, SV40-transformed
Description: Whole cell lysate (non-denatured)
Preparation Method: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
Quantity: 200 μg
Host Species: African Green Monkey
For Use With (Application): Western Blot
Lysis Buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Quality Control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer: In modified RIPA Lysis Buffer.
Content And Storage: Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Tissue:
Kidney, SV40-transformed
Description:
Whole cell lysate (non-denatured)
Preparation Method:
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
Quantity:
200 μg
Host Species:
African Green Monkey
For Use With (Application):
Western Blot
Lysis Buffer:
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Quality Control Testing:
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer:
In modified RIPA Lysis Buffer.
Content And Storage:
Store at -80°C. Aliquot to avoid repeated freezing and thawing.