BDB612069
BD NIH 3T3 + Vanadate + PDGF Lysate
Manufacturer: BD
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Tissue
Embryonic Fibroblast
Quantity
500 μg
Host Species
Mouse
Content And Storage
-20°C
Preparation Method
NIH 3T3 cells were Serum starved overnight and then incubated at room temperature for 10 min. in a final concentration of 1mM Sodium Vanadate, PDGF was then added at a final concentration of 5ng/mL and cells were incubated at 37°C
Storage Buffer
1mg/mL in SDS-PAGE Buffer: 62mM Tris pH 6.8, 2% SDS, 0.9% beta-mercaptoethanol, 0.003% Bromophenol Blue, 5% Glycerol
Concentration
1 mg/mL
Related Products
Description
- Can be rich in endogenously expressed proteins Convenient to use and provided in a denatured format allowing for immediate loading onto an SDS-PAGE gel
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Tissue: Embryonic Fibroblast
Quantity: 500 μg
Host Species: Mouse
Content And Storage: -20°C
Preparation Method: NIH 3T3 cells were Serum starved overnight and then incubated at room temperature for 10 min. in a final concentration of 1mM Sodium Vanadate, PDGF was then added at a final concentration of 5ng/mL and cells were incubated at 37°C
Storage Buffer: 1mg/mL in SDS-PAGE Buffer: 62mM Tris pH 6.8, 2% SDS, 0.9% beta-mercaptoethanol, 0.003% Bromophenol Blue, 5% Glycerol
Concentration: 1 mg/mL
Tissue:
Embryonic Fibroblast
Quantity:
500 μg
Host Species:
Mouse
Content And Storage:
-20°C
Preparation Method:
NIH 3T3 cells were Serum starved overnight and then incubated at room temperature for 10 min. in a final concentration of 1mM Sodium Vanadate, PDGF was then added at a final concentration of 5ng/mL and cells were incubated at 37°C
Storage Buffer:
1mg/mL in SDS-PAGE Buffer: 62mM Tris pH 6.8, 2% SDS, 0.9% beta-mercaptoethanol, 0.003% Bromophenol Blue, 5% Glycerol
Concentration:
1 mg/mL
Tissue: __
Quantity: 50 μg
Host Species: Mouse
Content And Storage: Store undiluted at -20°C.
Preparation Method: __
Storage Buffer: __
Concentration: 250μg/mL
Tissue:
__
Quantity:
50 μg
Host Species:
Mouse
Content And Storage:
Store undiluted at -20°C.
Preparation Method:
__
Storage Buffer:
__
Concentration:
250μg/mL
Tissue: __
Quantity: 150 μg
Host Species: Mouse
Content And Storage: Store undiluted at -20°C.
Preparation Method: __
Storage Buffer: __
Concentration: 250μg/mL
Tissue:
__
Quantity:
150 μg
Host Species:
Mouse
Content And Storage:
Store undiluted at -20°C.
Preparation Method:
__
Storage Buffer:
__
Concentration:
250μg/mL
Tissue: __
Quantity: 50 μg
Host Species: Mouse
Content And Storage: Store undiluted at -20°C.
Preparation Method: __
Storage Buffer: __
Concentration: 250μg/mL
Tissue:
__
Quantity:
50 μg
Host Species:
Mouse
Content And Storage:
Store undiluted at -20°C.
Preparation Method:
__
Storage Buffer:
__
Concentration:
250μg/mL