Thermo Scientific™ Pierce™ Cypridina Luciferase Flash Assay Kit

Description

• The Thermo Scientific™ Pierce Cypridina Luciferase Flash Assay Kit provides researchers with a highly sensitive assay for transcriptional activity of regulatory elements in mammalian cell culture media and whole cell lysate
• This Flash Assay Kit contains reagents for measuring the activity of Cypridina luciferase in mammalian cell culture media and lysates
• When used with Thermo Scientific Cypridina Luc Vectors, the kit provides an extremely sensitive bioluminescent reporter assay system for secreted or intracellular detection of promoter or pathway activity
• Cypridina luciferase is highly secreted into the cell culture media, allowing for live cell monitoring of reporter activity
• The signal produced by Cypridina is considerably greater than signal from either firefly or Renilla luciferases assayed under similar conditions
• Highlights: Sensitive – high sensitivity allows utilization of smaller numbers of cells Cost effective – sensitive assays result in decreased reagent consumption Secreted luciferase – allows real-time assays and kinetic studies without destroying cells Ideal for co-transfection – uses a different substrate than Renilla , Gaussia , or firefly luciferases Compatible – assay reagents compatible with other Cypridina or Vargula luciferases Time-saving – assays using secreted luciferases require minimal sample handling Automation-friendly – amenable to high throughput screens Convenient – contains a universal cell lysis buffer and optimized flash assay reagent Safe – allows one to perform non-radioactive assays Includes: Cell lysis buffer, reaction buffer and substrate Requires: Cypridina luciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers
• Recommended for: Promoter studies for analyzing cis - regulatory elements and trans -acting factors; Drug screening; siRNA and miRNA screening; Multiplexed assays to study off-target effects; Secretory pathway / protein localization reporter assays; Signal transduction pathway analysis; RNA splicing studies