Thermo Scientific™ Pierce™ Gaussia Luciferase Flash Assay Kit

Description

• The Thermo Scientific™ Pierce Gaussia Luciferase Flash Assay Kit provides researchers with the most sensitive assay for transcriptional activity of regulatory elements in mammalian cell culture media and whole cell lysate
• This Flash Assay Kit contains reagents for measuring the activity of Gaussia luciferase in mammalian cell culture media and lysates
• When used with Thermo Scientific Gaussia Luc Vectors, the kit provides an extremely sensitive bioluminescent reporter assay system for secreted or intracellular detection of promoter or pathway activity
• Gaussia luciferase is highly secreted into the cell culture media, allowing for live cell monitoring of reporter activity
• The signal produced by Gaussia is considerably greater than signal from either firefly or Renilla luciferases assayed under similar conditions
• Highlights: Sensitive – 1000 times greater sensitivity allows utilization of smaller numbers of cells Cost effective – highest sensitivity assays result in decreased reagent consumption Secreted luciferase – allows real-time assays and kinetic studies without destroying cells Time-saving – assays using secreted luciferases require minimal sample handling Automation-friendly – amenable to high throughput screens Convenient – contains a universal cell lysis buffer and optimized flash assay reagent Safe – allows one to perform non-radioactive assays Includes: Cell lysis buffer, reaction buffer and substrate Requires: Gaussia luciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers Recommended for: Promoter studies for analyzing cis - regulatory elements and trans -acting factors; Drug screening; siRNA and miRNA screening; Multiplexed assays to study off-target effects; Secretory pathway / protein localization reporter assays; Signal transduction pathway analysis; RNA splicing studies