Thermo Scientific™ Pierce™ Gaussia-Firefly Luciferase Dual Assay Kit

Description

• The Thermo Scientific™ Pierce Gaussia-Firefly Luciferase Dual Assay Kit provides the necessary reagents to simultaneously detect intracellular Gaussia and Red Firefly luciferase activity in mammalian whole cell lysates
• The Pierce Gaussia-Firefly Luciferase Dual Assay Kit is a highly sensitive assay that allows for the simultaneous detection of Gaussia and Red Firefly luciferase activity
• Gaussia luciferase acts as an experimental reporter with constitutively active red firefly as a normalization control
• This reporter-and-control combination enables simultaneous monitoring of experimental reporter and control luciferase activities in a single-read assay without the need for two-step addition of substrate reagents or quenching
• The assay working solution contains substrates for both luciferases, and the reactions occur simultaneously with flash-type kinetics
• The resulting luminescent signals are spectrally resolvable using filters
• In a single sample, researchers can assay transcriptional activity of regulatory elements, signal transduction pathways, and effects of activators or inhibitors
• Highlights: Simultaneous – concurrent, filter-based, wavelength-separated detection of two luciferase activities Sensitive – measure bright blue Gaussia and red firefly luciferase activities in the same sample Fast – no quenching step required, unlike in traditional sequential dual assays Multiplex – capable of quantitating two cellular activities in the same sample(s) Includes: Cell lysis buffer, reaction buffer, and substrates coelenterazine and D-luciferin Requires: Gaussia and Red Firefly luciferase reporters; Filter sets in the ranges of 425 to 525nm for Gaussia Luc and 615 to 675nm for Red Firefly Luc
• Luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers; Injector required for assessing more than 24 wells at a time
• Recommended for: Study two regulatory elements at the same time; Monitor two signaling pathways simultaneously; Enable studying more than one target per screen (e.g
• off-target effects)