Thermo Scientific™ Pierce™ Firefly Luc One-Step Glow Assay Kit

Description

• The Thermo Scientific Pierce Firefly One-Step Luc Glow Assay Kit measures firefly luciferase activity in mammalian cell culture using a single reagent-addition step, making it ideal for high-throughput screening (HTS) applications
• This glow-type luciferase assay kit is designed for use with cultured mammalian cells that are transfected with vectors expressing the appropriate firefly luciferase reporter enzyme
• Several features make the kit especially suitable for luminometers without injectors and for high-throughput screening (HTS) applications
• The convenient, one-step, homogenous protocol minimizes handling steps to support the use of automation
• Simply combine the supplied substrate solution and assay buffer to make a single working solution, and then add it to microplate wells containing the transfected, treated cells
• The stable signal output (glow) of this system provides flexibility with regard to incubation time
• When used with Thermo Scientific Firefly Luc Plasmids, the kit provides a highly sensitive bioluminescent reporter assay system for the detection of promoter or pathway activity
• Highlights: Sensitive - highly sensitive detection of firefly luciferase activity Convenient - one-step, mix-and-read protocol; no additional reagents required Stable - increased signal half-life and stability compared to flash assays Simple - does not require a luminometer with injectors Automation-friendly - amenable to high throughput assays Includes: Assay buffer and substrate solution (100X) Requires: Firefly luciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers
• Applications: Promoter studies for analyzing cis- and trans-acting regulatory elements Drug screening siRNA and miRNA screening Multiplexed assays to study off-target effects Protein localization reporter assays Signal transduction pathway analysis Product Details: Firefly luciferase is a 62kDa protein produced in nature by several species of the Lampyridae family of beetles, which includes the genera Photinus and Luciola
• Thermo Scientific Red Firefly Luciferase has an emission range of 560-700nm (λmax = 613nm)
• Bioluminescent signal from firefly luciferase originates from the oxidation of D-Luciferin
• Light output captured using a luminometer can be correlated with the amount of firefly luciferase protein produced and used to determine the activity of the promoter driving firefly luciferase expression
• Firefly luciferase bioluminescence provides researchers with a non-radioactive assay for measuring transcriptional activity of regulatory elements
• Firefly luciferase reporter activity can be used to determine promoter activity, to study upstream pathway activity in the cell, or to determine effect of activators or inhibitors.