Thermo Scientific™ Pierce™ Firefly Luciferase Flash Assay Kit

Description

• The Thermo Scientific™ Pierce Firefly Luciferase Flash Assay Kit provides reagents to measure Red firefly luciferase activity in whole cell producing a very strong flash signal which decays rapidly
• Thermo Scientific Pierce Firefly Luciferase Flash Assay Kit contains reagents to measure the activity of firefly luciferase in mammalian whole cell lysates
• Containing a convenient lysis buffer, assay buffer and firefly substrate, this kit provides a complete reagent system for the intracellular detection of promoter or pathway activity
• Light output resulting from the luciferase reaction is captured using a luminometer and can be correlated with the amount of firefly luciferase protein produced and used to determine the activity of the promoter driving firefly expression
• These reagents are optimized to work with Thermo Scientific Firefly Luc Plasmids; however, the Flash Assay Kit may be used to detect the activity of other firefly or ATP-dependent luciferases utilizing D-luciferin as a substrate
• Highlights: Highly sensitive detection of firefly luciferase activity Use with Thermo Scientific Pierce Firefly Luciferase Signal Enhancer for best results (maximum signal) Working solution stable for up to 2 months at -20°C Assay reagents compatible with other firefly luciferases Amenable to automation Contains a universal cell lysis buffer and optimized flash assay reagent Safe non-radioactive assay Includes: Cell lysis buffer, reaction buffer and substrate Requires: Firefly luciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers; For best results (maximum signal), use with Thermo Scientific Pierce Firefly Luciferase Signal Enhancer Recommended for: Promoter studies for analyzing cis - regulatory elements and trans -acting factors; Drug screening; siRNA and miRNA screening; Multiplexed assays to study off-target effects; Secretory pathway / protein localization reporter assays; Signal transduction pathway analysis; RNA splicing studies