Thermo Scientific™ Pierce™ Renilla Luciferase Flash Assay Kit

Description

• The Thermo Scientific™ Pierce Renilla Luciferase Flash Assay Kit provides researchers with a highly sensitive intracellular assay for transcriptional activity of regulatory elements in mammalian whole cell lysate
• This Flash Assay Kit contains reagents for measuring the activity of Renilla luciferase in mammalian cell lysates
• When used with Thermo Scientific Green Renilla Luc Plasmids, the kit provides an extremely sensitive bioluminescent reporter assay system for intracellular detection of promoter or pathway activity
• The signal produced by Green Renilla Luc is considerably greater than signal from either native firefly or Renilla luciferases assayed under similar conditions
• Light output captured using a luminometer can be correlated with the amount of Renilla luciferase protein produced and used to determine the activity of the promoter driving Renilla expression
• Highlights: Sensitive – greater sensitivity allows utilization of smaller numbers of cells Cost effective – highest sensitivity assays result in decreased reagent consumption Time-saving – assays require minimal sample handling Automation-friendly – amenable to high throughput screens Convenient – contains a universal cell lysis buffer and optimized flash assay reagent Safe – allows one to perform non-radioactive assays Includes: Cell lysis buffer, reaction buffer and substrate Requires: Renilla luciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers Recommended for: Promoter studies for analyzing cis - regulatory elements and trans -acting factors; Drug screening; siRNA and miRNA screening; Multiplexed assays to study off-target effects; Protein localization reporter assays; Signal transduction pathway analysis; RNA splicing studies