MilliporeSigma™ Calbiochem™ L3MBTL3 MBT Domain Blocker, UNC1215

Description

• A cell-permeable pyrrolidinyl-piperidinyl-benzamide that effectively competes against H4K20Me2 peptide for L3MBTL3 binding by targeting the first two L3MBTL3 MBT domains ( K d = 120nM in binding studies using recombinant 3MBT fragment), while exhibiting much reduced potency against L3MBTL1-H4K20Me1, 53BP1-H4K20Me2, MBTD1-H4K20Me1, or L3MBTL4-H2AK36Me1 interaction (IC 50 ≥2μM; [peptide] = 150nM)
• Shown to increase GFP-L3MBTL fusion nucleus mobility (10nM to 1μM) in HEK293 transfectants
• X-ray structural analysis reveals two 3MBT are bridged together by two UNC1215 molecules in a reciprocal fashion
• Please note that the molecular weight for this compound is batch-specific due to variable water content
• Please refer to the vial label or the certificate of analysis for the batch-specific molecular weight
• The molecular weight provided represents the baseline molecular weight without water.