PI88941
Thermo Scientific™ GlycoLink™ Immobilization Kit
Manufacturer: Thermo Scientific™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PI88941-Each-of-1 | In Stock | ₹ 90,246.00 |
PI88941 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 90,246.00
GST (18%): ₹ 16,244.28
Total Price: ₹ 1,06,490.28
Capacity (Metric)
1 to 10 mg of GlycoProtein Immobilization per Column
Content And Storage
Sufficient For: Immobilizing 1 to 10 mg of glycoprotein per column• GlycoLink Columns, 1 mL, 10 columns• GlycoLink Coupling Buffer, 2 x 100 mL• Aniline, 0.2 mL• Wash Buffer, 120 mL• Sodium meta-periodate, 0.5 g• Zeba Spin Desalting Columns, 5 mL, 10 columns• PBS Pack (makes 500mL), 1 pack• Column Accessories, 10 eaStore at 4°C.
Quantity
1 Kit
Stationary Phase
Hydrazide
Format
Spin Column
Description
GlycoLink Immobilization Kit
Product Line
GlycoLink™
Type
Immobilization Kit
Sufficient For
10 Columns
Column Type
Affinity, UltraLink™ Resin
Description
- Thermo Scientific™ GlycoLink Immobilization Kits provide affinity columns and reagents to immobilize glycoproteins through oxidized sugar groups for use in affinity-purification applications or studies of protein-protein interactions
- The GlycoLink Kits use hydrazide-activated resin and a special catalyst to prepare affinity columns with polyclonal antibodies, which typically have abundant carbohydrates (glycosylation) on their Fc portions
- Any glycosylated proteins (even monoclonal antibodies) that contain periodate-oxidizable carbohydrate groups can be immobilized with these kits to make reusable affinity columns
- Antibodies immobilized by this hydrazide immobilization chemistry have unobstructed antigen-binding sites and optimal purification capability
- Prepared columns with stable glycoproteins or antibodies can be regenerated and reused at least five times for affinity-purification without significant loss in binding capacity
- Highlights: High-capacity – immobilize 1 to 10mg of oxidized antibody or other glycoprotein per milliliter of resin Fast and efficient – immobilize in as few as 30 minutes; achieve at least 90% coupling of most glycoproteins in less than 4 hours (efficiency depends on the amount and type of glycosylation) Specific immobilization – the hydrazide-activated UltraLink Resin conjugates only to purified glycoproteins whose sugar (e.g., sialic acid) groups have been gently oxidized with periodate Stable attachment – resonance structure of the hydrazone bonds are sufficiently stable for multiple affinity purifications with one batch of prepared resin; no stabilization step is required Preserves antibody function – immobilizes IgG via the Fc region, thereby keeping both antigen binding sites available for capturing target Convenient sizes – choose from two complete 10-column kits: one with 1mL columns and one with 0.1mL columns UltraLink Hydrazide Resin: Ligand: hydrazide Support: Thermo Scientific UltraLink Biosupport Spacer arm: 22 atoms Loading: activated with at least 15μmol hydrazide per milliliter of settled resin Format: 50% resin slurry in 0.02% sodium azide Includes: Kits contain GlycoLink Columns; coupling buffer, coupling catalyst and wash buffer; oxidizing reagent; desalting columns, PBS buffer pack; and column accessories Recommended for: Purify antigens using covalently coupled antibodies; Determine binding partners for a glycoprotein of interest; Perform large-scale affinity purifications
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Capacity (Metric): 1 to 10 mg of GlycoProtein Immobilization per Column
Content And Storage: Sufficient For: Immobilizing 1 to 10 mg of glycoprotein per column• GlycoLink Columns, 1 mL, 10 columns• GlycoLink Coupling Buffer, 2 x 100 mL• Aniline, 0.2 mL• Wash Buffer, 120 mL• Sodium meta-periodate, 0.5 g• Zeba Spin Desalting Columns, 5 mL, 10 columns• PBS Pack (makes 500mL), 1 pack• Column Accessories, 10 eaStore at 4°C.
Quantity: 1 Kit
Stationary Phase: Hydrazide
Format: Spin Column
Description: GlycoLink Immobilization Kit
Product Line: GlycoLink™
Type: Immobilization Kit
Sufficient For: 10 Columns
Column Type: Affinity, UltraLink™ Resin
Capacity (Metric):
1 to 10 mg of GlycoProtein Immobilization per Column
Content And Storage:
Sufficient For: Immobilizing 1 to 10 mg of glycoprotein per column• GlycoLink Columns, 1 mL, 10 columns• GlycoLink Coupling Buffer, 2 x 100 mL• Aniline, 0.2 mL• Wash Buffer, 120 mL• Sodium meta-periodate, 0.5 g• Zeba Spin Desalting Columns, 5 mL, 10 columns• PBS Pack (makes 500mL), 1 pack• Column Accessories, 10 eaStore at 4°C.
Quantity:
1 Kit
Stationary Phase:
Hydrazide
Format:
Spin Column
Description:
GlycoLink Immobilization Kit
Product Line:
GlycoLink™
Type:
Immobilization Kit
Sufficient For:
10 Columns
Column Type:
Affinity, UltraLink™ Resin
Capacity (Metric): Immobilizing 50 to 500 μg of GlycoProtein per Column
Content And Storage: Sufficient For: Immobilizing 50 to 500 μg of glycoprotein per column• GlycoLink Columns, 0.1 mL, 10 columns• GlycoLink Coupling Buffer, 100 mL• Aniline, 0.2 mL• Wash Buffer, 60 mL• Sodium meta-periodate, 0.5 g• Zeba Spin Desalting Columns, 0.5 mL, 10 columns• PBS Pack (makes 500mL), 1 pack• Column Accessories, 10 eaStore at 4°C.
Quantity: 10 Columns
Stationary Phase: Hydrazide
Format: Kit, Microcentrifuge, Spin Column
Description: GlycoLink Micro Immobilization Kit
Product Line: GlycoLink™
Type: Immobilization Kit
Sufficient For: 10 Columns
Column Type: Affinity, UltraLink™ Resin
Capacity (Metric):
Immobilizing 50 to 500 μg of GlycoProtein per Column
Content And Storage:
Sufficient For: Immobilizing 50 to 500 μg of glycoprotein per column• GlycoLink Columns, 0.1 mL, 10 columns• GlycoLink Coupling Buffer, 100 mL• Aniline, 0.2 mL• Wash Buffer, 60 mL• Sodium meta-periodate, 0.5 g• Zeba Spin Desalting Columns, 0.5 mL, 10 columns• PBS Pack (makes 500mL), 1 pack• Column Accessories, 10 eaStore at 4°C.
Quantity:
10 Columns
Stationary Phase:
Hydrazide
Format:
Kit, Microcentrifuge, Spin Column
Description:
GlycoLink Micro Immobilization Kit
Product Line:
GlycoLink™
Type:
Immobilization Kit
Sufficient For:
10 Columns
Column Type:
Affinity, UltraLink™ Resin
Capacity (Metric): 2 to 10 μg Oxidized Antibody or Glycoprotein Coupling to 20 μL Resin
Content And Storage: Sufficient For: 25 IP/Co-IP reactions, each using 20 μL antibody-coupled resin• UltraLink Hydrazide Resin, 0.5 mL• GlycoLink Coupling Buffer, 50 mL• Aniline, 0.2 mL• Sodium meta-periodate, 0.5 g• Wash Buffer, 60 mL• Zeba Spin Desalting Columns,0.5 mL, 25 columns• IP Lysis/Wash Buffer, 50 mL• PBS Pack (makes 500 mL), 1 pack• Elution Buffer, pH 2.8, 50 mL• Lane Marker Sample Buffer, Non-reducing, (5X), 5 mL• Pierce Spin Columns-Screw Caps, 25 columns• Collection Tubes, 2 mL, 100 tubesStore at 4°C.
Quantity: 1 Kit
Stationary Phase: __
Format: Kit
Description: __
Product Line: GlycoLink™
Type: Immunoprecipitation/Co-Immunoprecipitation Kit
Sufficient For: 25 Reactions
Column Type: __
Capacity (Metric):
2 to 10 μg Oxidized Antibody or Glycoprotein Coupling to 20 μL Resin
Content And Storage:
Sufficient For: 25 IP/Co-IP reactions, each using 20 μL antibody-coupled resin• UltraLink Hydrazide Resin, 0.5 mL• GlycoLink Coupling Buffer, 50 mL• Aniline, 0.2 mL• Sodium meta-periodate, 0.5 g• Wash Buffer, 60 mL• Zeba Spin Desalting Columns,0.5 mL, 25 columns• IP Lysis/Wash Buffer, 50 mL• PBS Pack (makes 500 mL), 1 pack• Elution Buffer, pH 2.8, 50 mL• Lane Marker Sample Buffer, Non-reducing, (5X), 5 mL• Pierce Spin Columns-Screw Caps, 25 columns• Collection Tubes, 2 mL, 100 tubesStore at 4°C.
Quantity:
1 Kit
Stationary Phase:
__
Format:
Kit
Description:
__
Product Line:
GlycoLink™
Type:
Immunoprecipitation/Co-Immunoprecipitation Kit
Sufficient For:
25 Reactions
Column Type:
__
Capacity (Metric): __
Content And Storage: Store at 4°C.
Quantity: 100 mL
Stationary Phase: __
Format: __
Description: __
Product Line: GlycoLink™
Type: __
Sufficient For: __
Column Type: __
Capacity (Metric):
__
Content And Storage:
Store at 4°C.
Quantity:
100 mL
Stationary Phase:
__
Format:
__
Description:
__
Product Line:
GlycoLink™
Type:
__
Sufficient For:
__
Column Type:
__