PI44667
Thermo Scientific™ Pierce™ Protein A IgG Purification Kit, 1 mL
Manufacturer: Thermo Scientific™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Pack of 1 | PI44667-Pack-of-1 | In Stock | ₹ 72,298.20 |
PI44667 - Pack of 1
In Stock
Quantity
1
Base Price: ₹ 72,298.20
GST (18%): ₹ 13,013.676
Total Price: ₹ 85,311.876
Product Type
IgG Purification Kit
Column Type
Agarose Resin, Affinity
Stationary Phase
Protein A
Sufficient For
6 to 8 mg Mouse IgG per Column
Quantity
5 Column Kit
Product Line
Pierce™
Format
Kit, 15 mL Conical Spin Column
Purification Target
IgG Antibodies
Content And Storage
Sufficient For: Purifying 6 to 8 mg mouse IgG per column• Protein A Columns, 1 mL, 5 columns• Protein A IgG Binding Buffer, 1 L• IgG Elution Buffer, 500 mL• Excellulose Desalting Columns, 5 mL, 5 columnsStore at 4°C.
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Description
- Perform a variety of antibody affinity purification methods with Thermo Scientific™ Pierce Protein A Agarose, a standard-capacity Protein A beaded agarose resin
- Pierce Protein A Agarose consists of purified native Protein A that has been covalently immobilized onto high-quality crosslinked 6% beaded agarose
- The agarose beads have physical and chemical properties that enable them to be used in a variety of batch- or column-type affinity purification procedure
- Several package formats are offered: 5mL and 25mL bottles of resin slurry, 1mL pre-packed columns and a complete IgG purification kit
- Protein A is a bacteria-derived protein that binds with high affinity and specificity to the Fc portion of antibodies, especially those of the IgG class
- Compared to its alternative (Protein G), Protein A provides the highest binding capacity for subclasses of IgG from rabbits, pigs, dogs and cats
- Protein A also has higher binding capacity for human IgG, except for IgG3, which it binds weakly
- Protein A also binds weakly to mouse IgG1 and is not recommended for most applications with that antibody isotype
- Highlights: Native Protein A – immobilized Protein A is ideal for polyclonal IgG purification from human, rabbit, pig, dog or cat serum Agarose resin – support is crosslinked 6% beaded agarose (CL-6B), the most popular resin for protein affinity purification methods Inert and stable – superior manufacturing method immobilizes Protein A by charge-free, leach-resistant covalent bonds, resulting in low nonspecific binding and enabling multiple uses without decline in yield Standard capacity – Pierce Protein A Agarose has a normal load of immobilized Protein A, providing a binding capacity of 12 to 19mg human IgG/mL resin (approx
- 6 to 8mg mouse IgG/mL resin) Properties of crosslinked 6% beaded agarose (CL-6B): Support pH Stability: 2 to 14 (short term); 3 to 13 (long term) Average Particle Size: 45 to 165 microns Exclusion Limit: 10,000 to 4,000,000 daltons Maximum Volumetric Flow Rate: approx
- 1mL/minute (for 1cm diameter column) Maximum Linear Velocity: 30cm per hour Maximum Pressure: less than 25psi (1.5 bar), defined as the maximum pressure drop across a column that the resin can withstand (Note: The indicated gauge pressure of a liquid chromatography apparatus may be measuring the total system pressure rather than the pressure drop across the column.) Available Formats: Resin Slurries: crosslinked 6% beaded agarose; 5mL and 25mL bottles Spin Columns: 1mL columns Purification Kit: complete kit containing five 1mL columns Bulk and Custom: let us know your needs for bulk Pierce Protein A Agarose Includes: Kit contains Protein A Column (1mL each), Protein A IgG Binding Buffer, IgG Elution Buffer and desalting columns
Compare Similar Items
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Product Type: IgG Purification Kit
Column Type: Agarose Resin, Affinity
Stationary Phase: Protein A
Sufficient For: 6 to 8 mg Mouse IgG per Column
Quantity: 5 Column Kit
Product Line: Pierce™
Format: Kit, 15 mL Conical Spin Column
Purification Target: IgG Antibodies
Content And Storage: Sufficient For: Purifying 6 to 8 mg mouse IgG per column• Protein A Columns, 1 mL, 5 columns• Protein A IgG Binding Buffer, 1 L• IgG Elution Buffer, 500 mL• Excellulose Desalting Columns, 5 mL, 5 columnsStore at 4°C.
Product Type:
IgG Purification Kit
Column Type:
Agarose Resin, Affinity
Stationary Phase:
Protein A
Sufficient For:
6 to 8 mg Mouse IgG per Column
Quantity:
5 Column Kit
Product Line:
Pierce™
Format:
Kit, 15 mL Conical Spin Column
Purification Target:
IgG Antibodies
Content And Storage:
Sufficient For: Purifying 6 to 8 mg mouse IgG per column• Protein A Columns, 1 mL, 5 columns• Protein A IgG Binding Buffer, 1 L• IgG Elution Buffer, 500 mL• Excellulose Desalting Columns, 5 mL, 5 columnsStore at 4°C.
Product Type: __
Column Type: __
Stationary Phase: __
Sufficient For: __
Quantity: 10 Antibody Samples Each Containing 25 to 250 μg IgG
Product Line: Pierce™
Format: __
Purification Target: __
Content And Storage: Sufficient For: 10 antibody samples, each containing 25 to 250 μg IgG• Pierce Ficin Agarose, 0.8 mL• Cysteine-HCl, 1 g• Mouse IgG1 Digestion Buffer, 120 mL• NAb Protein A Plus Spin Columns, 0.2 mL, 2 columns• Protein A Binding Buffer, 50 mL• IgG Elution Buffer, 50 mL• Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, 10 columns• Spin Columns, 0.8 mL, 10 columns• Microcentrifuge tubes, 2 mL, 30 tubesStore at 4–8°C.
Product Type:
__
Column Type:
__
Stationary Phase:
__
Sufficient For:
__
Quantity:
10 Antibody Samples Each Containing 25 to 250 μg IgG
Product Line:
Pierce™
Format:
__
Purification Target:
__
Content And Storage:
Sufficient For: 10 antibody samples, each containing 25 to 250 μg IgG• Pierce Ficin Agarose, 0.8 mL• Cysteine-HCl, 1 g• Mouse IgG1 Digestion Buffer, 120 mL• NAb Protein A Plus Spin Columns, 0.2 mL, 2 columns• Protein A Binding Buffer, 50 mL• IgG Elution Buffer, 50 mL• Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, 10 columns• Spin Columns, 0.8 mL, 10 columns• Microcentrifuge tubes, 2 mL, 30 tubesStore at 4–8°C.
Product Type: __
Column Type: __
Stationary Phase: __
Sufficient For: __
Quantity: 10 Antibody Samples Each Containing 25 to 250 μg IgG
Product Line: Pierce™
Format: __
Purification Target: __
Content And Storage: Sufficient For: 10 antibody samples, each containing 25 to 250 μg IgG• Pierce Papain Agarose, 0.5 mL• Cysteine-HCl, 1 g• Fab Digestion Buffer, 55 mL• NAb Protein A Plus Spin Columns, 0.2 mL, 2 columns• PBS Packs (each makes 500 mL), 2 packs• IgG Elution Buffer, 50 mL• Zeba Desalt Spin Columns, 7K MWCO, 0.5 mL, 10 columns• Spin Columns, 0.8 mL, 10 columns• Microcentrifuge Tubes, 2 mL, 30 tubesStore at 4–8°C.
Product Type:
__
Column Type:
__
Stationary Phase:
__
Sufficient For:
__
Quantity:
10 Antibody Samples Each Containing 25 to 250 μg IgG
Product Line:
Pierce™
Format:
__
Purification Target:
__
Content And Storage:
Sufficient For: 10 antibody samples, each containing 25 to 250 μg IgG• Pierce Papain Agarose, 0.5 mL• Cysteine-HCl, 1 g• Fab Digestion Buffer, 55 mL• NAb Protein A Plus Spin Columns, 0.2 mL, 2 columns• PBS Packs (each makes 500 mL), 2 packs• IgG Elution Buffer, 50 mL• Zeba Desalt Spin Columns, 7K MWCO, 0.5 mL, 10 columns• Spin Columns, 0.8 mL, 10 columns• Microcentrifuge Tubes, 2 mL, 30 tubesStore at 4–8°C.
Product Type: __
Column Type: __
Stationary Phase: __
Sufficient For: __
Quantity: 10 Antibody Samples, each containing 25 to 250 μg IgG
Product Line: Pierce™
Format: __
Purification Target: __
Content And Storage: Sufficient For: 10 antibody samples, each containing 25 to 250 μg IgG• Pierce Pepsin Agarose, 0.5 mL• F(ab')2 Digestion Buffer, 55 mL• NAb Protein A Plus Spin Columns, 0.2 mL, 2 columns• PBS Packs (each makes 500 mL), 2 packs• IgG Elution Buffer, 50 mL• Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, 10 columns• Spin Columns, 0.8 mL, 10 columns• Microcentrifuge Tubes, 2 mL, 30 tubesStore at 4°C
Product Type:
__
Column Type:
__
Stationary Phase:
__
Sufficient For:
__
Quantity:
10 Antibody Samples, each containing 25 to 250 μg IgG
Product Line:
Pierce™
Format:
__
Purification Target:
__
Content And Storage:
Sufficient For: 10 antibody samples, each containing 25 to 250 μg IgG• Pierce Pepsin Agarose, 0.5 mL• F(ab')2 Digestion Buffer, 55 mL• NAb Protein A Plus Spin Columns, 0.2 mL, 2 columns• PBS Packs (each makes 500 mL), 2 packs• IgG Elution Buffer, 50 mL• Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, 10 columns• Spin Columns, 0.8 mL, 10 columns• Microcentrifuge Tubes, 2 mL, 30 tubesStore at 4°C